Detailed information of TA system TA075623

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 4636416..4637074
• Replicon: chromosome
• Accession: NZ_CP020882
• Organism: Xanthomonas citri pv. citri strain TX160042

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q8PRH3
• Locus tag: B7L65_RS21095
• Protein ID: WP_011053002.1
• Coordinates: 4636673..4637074 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q3C002
• Locus tag: B7L65_RS21090
• Protein ID: WP_005917852.1
• Coordinates: 4636416..4636676 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7L65_RS21075	4635034..4635622	+	589	Protein_3916	IS3 family transposase	-
B7L65_RS21080	4635653..4636225	-	573	WP_011053004.1	recombinase family protein	-
B7L65_RS21090	4636416..4636676	+	261	WP_005917852.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
B7L65_RS21095	4636673..4637074	+	402	WP_011053002.1	type II toxin-antitoxin system VapC family toxin	Toxin
B7L65_RS21100	4637099..4637362	+	264	WP_005917846.1	hypothetical protein	-
B7L65_RS21105	4637369..4638556	+	1188	WP_011053001.1	zeta toxin family protein	-
B7L65_RS21110	4639173..4640183	+	1011	WP_015471495.1	IS21-like element ISXci1 family transposase	-
B7L65_RS21115	4640183..4640965	+	783	WP_015471496.1	IS21-like element ISXci1 family helper ATPase IstB	-
B7L65_RS21120	4641101..4641304	-	204	Protein_3924	PAS domain-containing protein	-
B7L65_RS21125	4641389..4641757	+	369	WP_011052155.1	response regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4635037..4665627	30590
	-	inside	IScluster/Tn	-	-	4635037..4640965	5928

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-122)

Antitoxin

• SpoVT-AbrB domain (8-52)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14627.05 Da
• Isoelectric Point: 7.2785

>T75623 WP_011053002.1 NZ_CP020882:4636673-4637074 [Xanthomonas citri pv. citri]
MKRYMLDTNTVSHLVKSHPAVSRRVIEVPMTALCMSAITGGELMFGLAKVPDAKRLQQAVMELLRRVDVLPWDGAVMERY
GSVRADLEKQGKALGSLDMLIAAHALETDSVLVTNDAAFSRVVGLTVEDWTRS

Nucleotide

• Length: 402 bp

>T75623 NZ_CP020882:4636673-4637074 [Xanthomonas citri pv. citri]
ATGAAGCGGTACATGCTTGATACCAACACCGTGAGCCATCTCGTCAAGAGCCATCCGGCTGTCTCTCGACGGGTCATTGA
AGTGCCTATGACTGCGCTATGTATGTCCGCAATTACCGGTGGGGAGCTGATGTTCGGCTTGGCTAAGGTGCCAGACGCCA
AACGCCTTCAGCAAGCCGTCATGGAACTCCTGCGGCGCGTGGATGTGCTGCCTTGGGATGGCGCGGTAATGGAGCGTTAC
GGAAGCGTAAGAGCGGACCTAGAAAAGCAAGGTAAAGCGCTTGGGTCGCTCGACATGCTTATCGCAGCACATGCACTGGA
AACGGATTCTGTCCTCGTTACCAATGACGCCGCATTTAGTCGTGTTGTTGGTTTGACCGTGGAAGATTGGACACGCAGCT
AA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9770.95 Da
• Isoelectric Point: 5.0941

>AT75623 WP_005917852.1 NZ_CP020882:4636416-4636676 [Xanthomonas citri pv. citri]
VSQVAKLFINGRSQAVRLPAAYRFTEKEVFIRQDPETGDVILSRKPATWEDFFIALKSADVPANFLDAAERNQGTQDRDP
FAGWRE

Nucleotide

• Length: 261 bp

>AT75623 NZ_CP020882:4636416-4636676 [Xanthomonas citri pv. citri]
GTGAGCCAAGTTGCCAAGCTGTTCATCAATGGCCGCAGCCAGGCAGTCCGCTTGCCTGCCGCCTATCGCTTTACCGAGAA
AGAGGTTTTCATTCGCCAAGACCCGGAGACCGGTGACGTCATCCTGTCTCGGAAGCCTGCAACGTGGGAGGACTTCTTTA
TCGCACTCAAGAGCGCAGATGTGCCTGCTAATTTTCTAGATGCCGCCGAGCGCAATCAGGGCACGCAGGACCGTGATCCT
TTTGCGGGATGGCGCGAATGA

Structures

Toxin

• AlphaFold DB: Q8PRH3

Antitoxin

• AlphaFold DB: Q3C002