Detailed information of TA system TA075622

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 4613906..4614495
• Replicon: chromosome
• Accession: NZ_CP020882
• Organism: Xanthomonas citri pv. citri strain TX160042

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: M4U123
• Locus tag: B7L65_RS20975
• Protein ID: WP_015463824.1
• Coordinates: 4613906..4614238 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: M4UHU6
• Locus tag: B7L65_RS20980
• Protein ID: WP_011052949.1
• Coordinates: 4614238..4614495 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7L65_RS26155	4612386..4612607	-	222	WP_011052944.1	hypothetical protein	-
B7L65_RS20965	4612897..4613433	-	537	WP_015463823.1	plasmid pRiA4b ORF-3 family protein	-
B7L65_RS20970	4613423..4613635	-	213	WP_011052947.1	hypothetical protein	-
B7L65_RS25810	4613632..4613883	-	252	WP_125459571.1	hypothetical protein	-
B7L65_RS20975	4613906..4614238	-	333	WP_015463824.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
B7L65_RS20980	4614238..4614495	-	258	WP_011052949.1	antitoxin	Antitoxin
B7L65_RS20985	4614701..4615303	+	603	WP_011052950.1	recombinase family protein	-
B7L65_RS20990	4615287..4615591	+	305	Protein_3901	Tn3 family transposase	-
B7L65_RS20995	4615758..4618718	-	2961	WP_015472939.1	Tn3 family transposase	-
B7L65_RS21000	4618763..4619188	-	426	Protein_3903	tyrosine-type recombinase/integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11707.46 Da
• Isoelectric Point: 9.1975

>T75622 WP_015463824.1 NZ_CP020882:c4614238-4613906 [Xanthomonas citri pv. citri]
MDRGDVYLVSLDPTSGHEQQGTRPVLIVSPSAFNRLTKTPVVLPITSGGNFARTAGFTVSLMGAGTNTTGVVRCDQPRAL
DLASRRARKLETVPSPIMDEVLAKLSAILE

Nucleotide

• Length: 333 bp

>T75622 NZ_CP020882:c4614238-4613906 [Xanthomonas citri pv. citri]
ATGGATCGCGGCGATGTCTATTTGGTTTCGCTCGATCCCACGTCCGGCCATGAACAACAGGGCACGCGCCCGGTGCTGAT
TGTGTCGCCAAGCGCGTTCAACCGCCTGACGAAAACACCGGTCGTGCTGCCGATCACCAGTGGGGGTAACTTCGCCCGCA
CGGCAGGCTTTACCGTGTCGCTCATGGGTGCGGGTACCAACACAACTGGCGTAGTTCGCTGCGATCAGCCCCGCGCTCTC
GATCTAGCCTCGCGGCGCGCGCGCAAGCTGGAAACCGTGCCGTCCCCGATCATGGATGAGGTATTAGCGAAGCTGAGCGC
GATCCTTGAGTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9242.70 Da
• Isoelectric Point: 4.7164

>AT75622 WP_011052949.1 NZ_CP020882:c4614495-4614238 [Xanthomonas citri pv. citri]
MHTTNLRKVGGSIMLAVPPAILDMLRLRAGATVGLAVDHGRLVIEPTLRPYYSLDELLAQCDASAELSTEDREWLDAKPV
GSELL

Nucleotide

• Length: 258 bp

>AT75622 NZ_CP020882:c4614495-4614238 [Xanthomonas citri pv. citri]
ATGCATACGACCAATCTGCGTAAGGTCGGTGGCTCGATCATGTTGGCTGTCCCGCCCGCCATTCTGGATATGCTGCGTCT
GCGTGCCGGCGCTACCGTTGGCTTGGCCGTCGATCATGGTCGCCTGGTGATCGAACCCACGCTGCGTCCGTACTACAGCC
TGGATGAGCTGCTGGCCCAATGCGACGCATCCGCCGAACTCTCCACCGAAGATCGGGAATGGCTCGACGCCAAGCCTGTT
GGCAGCGAGCTGCTGTGA

Structures

Toxin

• AlphaFold DB: M4U123

Antitoxin

• AlphaFold DB: M4UHU6