Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/MazF(toxin)
Location 275754..276390 Replicon chromosome
Accession NZ_CP020880
Organism Bacillus horikoshii strain 20a

Toxin (Protein)


Gene name mazF Uniprot ID A0A0K6GQX7
Locus tag B4U37_RS01440 Protein ID WP_010195163.1
Coordinates 276040..276390 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A0N0EA03
Locus tag B4U37_RS01435 Protein ID WP_010195162.1
Coordinates 275754..276035 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B4U37_RS01415 271866..272579 - 714 WP_010195154.1 rhomboid family intramembrane serine protease -
B4U37_RS01420 272698..273063 + 366 WP_088020081.1 holo-ACP synthase -
B4U37_RS01425 273226..274248 + 1023 WP_010195160.1 outer membrane lipoprotein carrier protein LolA -
B4U37_RS01430 274439..275608 + 1170 WP_088016777.1 alanine racemase -
B4U37_RS01435 275754..276035 + 282 WP_010195162.1 hypothetical protein Antitoxin
B4U37_RS01440 276040..276390 + 351 WP_010195163.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
B4U37_RS01445 276688..277518 + 831 WP_010195164.1 STAS domain-containing protein -
B4U37_RS01450 277515..277871 + 357 WP_010195165.1 STAS domain-containing protein -
B4U37_RS01455 277875..278276 + 402 WP_010195167.1 anti-sigma regulatory factor -
B4U37_RS01460 278292..279299 + 1008 WP_010195168.1 PP2C family protein-serine/threonine phosphatase -
B4U37_RS01465 279376..279684 + 309 WP_010195171.1 STAS domain-containing protein -
B4U37_RS01470 279733..280209 + 477 WP_010195173.1 anti-sigma B factor RsbW -
B4U37_RS01475 280184..280969 + 786 WP_010195175.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13006.03 Da        Isoelectric Point: 4.8891

>T75618 WP_010195163.1 NZ_CP020880:276040-276390 [Bacillus horikoshii]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDEEMMDKVDEALQISLGLIDF

Download         Length: 351 bp

>T75618 NZ_CP020880:276040-276390 [Bacillus horikoshii]
TTGATTGTCAAACGCGGTGACGTTTATTTTGCTGATTTATCCCCGGTTGTTGGGTCTGAGCAAGGAGGCGTTCGACCAGT
ACTTGTCATCCAAAATGATATCGGAAATCGATTTAGCCCGACCGTTATAGTAGCTGCCATTACTGCTCAAATCCAAAAAG
CCAAACTCCCAACACATGTAGAGATCGATGCGAAGCGTTATGGTTTTGAACGTGACTCTGTTATCCTGCTCGAACAAATT
CGAACAATTGATAAACAAAGATTAACGGACAAAATTACTCATCTTGACGAAGAGATGATGGACAAAGTGGATGAGGCGCT
TCAAATAAGCTTAGGACTTATTGACTTTTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10717.21 Da        Isoelectric Point: 7.0161

>AT75618 WP_010195162.1 NZ_CP020880:275754-276035 [Bacillus horikoshii]
VSESSATTEILIRLPQNLVSELDGLVKQENGNRNELIFQATKMYLRERKKRQIRESMRRGYMEMSKINLNIASEAFLAEY
EAEHTVERLVSGG

Download         Length: 282 bp

>AT75618 NZ_CP020880:275754-276035 [Bacillus horikoshii]
GTGTCTGAATCCAGCGCAACAACTGAAATCTTGATTCGATTACCTCAAAACTTAGTGTCGGAATTAGATGGTTTGGTAAA
ACAAGAAAACGGCAATCGAAACGAATTAATCTTTCAAGCAACAAAGATGTATCTTCGCGAGCGCAAAAAGCGTCAAATTC
GCGAGTCCATGAGACGTGGCTACATGGAAATGTCTAAAATCAATCTAAACATTGCATCTGAAGCTTTCTTGGCTGAATAT
GAGGCGGAGCATACGGTTGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K6GQX7

Antitoxin

Source ID Structure
AlphaFold DB A0A0N0EA03

References