Detailed information of TA system TA075617

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1681464..1682101
• Replicon: chromosome
• Accession: NZ_CP020874
• Organism: Bacillus velezensis strain GYL4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: B9C53_RS07665
• Protein ID: WP_003156187.1
• Coordinates: 1681464..1681814 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: B9C53_RS07670
• Protein ID: WP_003156188.1
• Coordinates: 1681820..1682101 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B9C53_RS07625	1676504..1677106	-	603	WP_007410234.1	SpoIIE family protein phosphatase	-
B9C53_RS07630	1677106..1677894	-	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
B9C53_RS07635	1677860..1678342	-	483	WP_007609591.1	anti-sigma B factor RsbW	-
B9C53_RS07640	1678339..1678668	-	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
B9C53_RS07645	1678732..1679739	-	1008	WP_007609589.1	PP2C family protein-serine/threonine phosphatase	-
B9C53_RS07650	1679751..1680152	-	402	WP_003156178.1	anti-sigma regulatory factor	-
B9C53_RS07655	1680155..1680520	-	366	WP_007609584.1	RsbT antagonist protein RsbS	-
B9C53_RS07660	1680525..1681346	-	822	WP_003156182.1	STAS domain-containing protein	-
B9C53_RS07665	1681464..1681814	-	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
B9C53_RS07670	1681820..1682101	-	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
B9C53_RS07675	1682221..1683390	-	1170	WP_038457028.1	alanine racemase	-
B9C53_RS07680	1683507..1684514	-	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
B9C53_RS07685	1684679..1685044	-	366	WP_007609580.1	holo-ACP synthase	-
B9C53_RS07690	1685137..1685736	+	600	WP_003156193.1	rhomboid family intramembrane serine protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T75617 WP_003156187.1 NZ_CP020874:c1681814-1681464 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T75617 NZ_CP020874:c1681814-1681464 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT75617 WP_003156188.1 NZ_CP020874:c1682101-1681820 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT75617 NZ_CP020874:c1682101-1681820 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5