Detailed information of TA system TA075603

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DhiAT/dnstrm_HI1420(antitoxin)
• Location: 1699528..1700284
• Replicon: chromosome
• Accession: NZ_CP020872
• Organism: Dickeya fangzhongdai strain PA1

Toxin (Protein)

• Gene name: dhiT
• Uniprot ID: -
• Locus tag: B6N31_RS07535
• Protein ID: WP_107759008.1
• Coordinates: 1699528..1699785 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: dhiA
• Uniprot ID: -
• Locus tag: B6N31_RS07540
• Protein ID: WP_121479931.1
• Coordinates: 1699793..1700284 (+)
• Length: 164 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B6N31_RS07525	1696303..1698453	-	2151	WP_049854965.1	formate dehydrogenase subunit alpha	-
B6N31_RS07530	1698488..1699030	-	543	WP_013317225.1	4Fe-4S dicluster domain-containing protein	-
B6N31_RS07535	1699528..1699785	+	258	WP_107759008.1	DUF4160 domain-containing protein	Toxin
B6N31_RS07540	1699793..1700284	+	492	WP_121479931.1	addiction module antitoxin	Antitoxin
B6N31_RS07545	1700684..1701106	+	423	WP_121479932.1	TIR domain-containing protein	-
B6N31_RS07550	1701284..1702516	+	1233	WP_121479933.1	DUF4071 domain-containing protein	-
B6N31_RS07555	1702579..1703076	-	498	WP_038918521.1	hydrogenase maturation peptidase HycI	-
B6N31_RS07560	1703069..1703467	-	399	WP_121479934.1	formate hydrogenlyase maturation HycH family protein	-
B6N31_RS07565	1703464..1704255	-	792	WP_038918522.1	NADH-quinone oxidoreductase subunit B family protein	-
B6N31_RS07570	1704252..1704821	-	570	WP_039691199.1	hydrogenase 4 subunit H	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF4160 (9-74)

Antitoxin

• Uncharacterized protein HI1420 (79-157)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9995.55 Da
• Isoelectric Point: 8.1489

>T75603 WP_107759008.1 NZ_CP020872:1699528-1699785 [Dickeya fangzhongdai]
MPEIDRLAGLIFHLYFYDDERHHLPHVHVCYGNHELIISIRTGTEIEGYLPNNKRKMALAHIRAHREQLLSMWERAVVGR
NPGKL

Nucleotide

• Length: 258 bp

>T75603 NZ_CP020872:1699528-1699785 [Dickeya fangzhongdai]
ATGCCTGAGATTGACAGACTGGCAGGGCTAATTTTCCATTTGTACTTTTACGATGATGAACGGCACCACTTGCCGCATGT
GCATGTCTGCTACGGCAACCATGAGCTGATCATCAGTATTCGTACTGGAACAGAGATCGAAGGATATCTGCCGAATAACA
AGCGCAAGATGGCGCTTGCGCATATCAGAGCCCATCGGGAACAACTGCTGAGTATGTGGGAGCGGGCGGTAGTGGGGAGA
AATCCGGGAAAATTGTGA

Antitoxin

Protein

• Length: 164 a.a.
• Molecular weight: 18069.63 Da
• Isoelectric Point: 6.6448

>AT75603 WP_121479931.1 NZ_CP020872:1699793-1700284 [Dickeya fangzhongdai]
MHKIVEVDVIGDFSLRLTYEDGLVCDINLYQYASSPVFSNASLFIKFGLLPDGSLEWSPDIKISASELRKKGEYVSHTLA
VREKNFADVISRAFYDAVIENRPEILQAALKIAVEKHGNSTVAKEAGAKSRTSLYKSLNKNTHVSLATVVSLAHTVLQLE
EKE

Nucleotide

• Length: 492 bp

>AT75603 NZ_CP020872:1699793-1700284 [Dickeya fangzhongdai]
ATGCATAAAATCGTGGAAGTTGACGTGATTGGCGACTTTTCGTTGCGCCTGACCTATGAGGATGGTTTGGTTTGCGACAT
CAATCTTTATCAATATGCCTCCAGCCCGGTATTTTCGAACGCCAGCCTATTTATCAAGTTTGGGCTGCTGCCGGATGGTT
CGCTGGAATGGTCGCCGGATATTAAGATTTCCGCGTCGGAATTGCGGAAAAAAGGGGAGTATGTCAGCCACACGCTTGCG
GTGCGAGAAAAGAACTTCGCTGATGTCATCTCTCGCGCCTTCTATGATGCCGTGATTGAAAACCGGCCGGAAATTCTGCA
AGCGGCGTTGAAAATCGCGGTGGAAAAACATGGCAACAGCACAGTAGCCAAAGAGGCGGGGGCGAAAAGTCGCACCAGTC
TGTATAAGTCGCTGAATAAAAATACCCATGTCAGTCTTGCGACGGTGGTCAGCCTGGCGCATACCGTACTGCAACTGGAA
GAGAAAGAATAA