Detailed information of TA system TA075598

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 431649..432685
• Replicon: chromosome
• Accession: NZ_CP020872
• Organism: Dickeya fangzhongdai strain PA1

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: B6N31_RS02010
• Protein ID: WP_038917617.1
• Coordinates: 432110..432685 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: B6N31_RS02005
• Protein ID: WP_038917616.1
• Coordinates: 431649..432110 (+)
• Length: 154 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B6N31_RS01995	426941..427234	+	294	WP_038917614.1	type I-E CRISPR-associated endoribonuclease Cas2	-
B6N31_RS02000	429424..431100	-	1677	WP_121479587.1	MCP four helix bundle domain-containing protein	-
B6N31_RS02005	431649..432110	+	462	WP_038917616.1	helix-turn-helix domain-containing protein	Antitoxin
B6N31_RS02010	432110..432685	+	576	WP_038917617.1	PIN domain-containing protein	Toxin
B6N31_RS02015	432909..433361	+	453	WP_038917619.1	YhbP family protein	-
B6N31_RS02020	433473..433937	-	465	WP_038917620.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
B6N31_RS02025	434036..436174	-	2139	WP_121479588.1	anaerobic ribonucleoside-triphosphate reductase	-
B6N31_RS02030	436554..437462	+	909	WP_121479589.1	homocysteine S-methyltransferase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-115)

Antitoxin

• SinI-like, DNA-binding domain (84-131)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21629.93 Da
• Isoelectric Point: 4.8531

>T75598 WP_038917617.1 NZ_CP020872:432110-432685 [Dickeya fangzhongdai]
MTHTPYPVVLDACVIYPSLLRDLLIHLGLTGLYQPKWTAIIEDEWQRNLLLNRPDLTPEQISRTRELMNQAIPDAMITGF
EALIPGITLPDPDDQHVVAAAIRSNAEIIVTFNLKDFPHPEIDNFGIEALHPDDFITDLLDLNQALVLSAVSRQRHSMRK
PPKSVDEYLDALLRQSLPQTVKELSKFHALI

Nucleotide

• Length: 576 bp

>T75598 NZ_CP020872:432110-432685 [Dickeya fangzhongdai]
ATGACCCATACACCTTATCCTGTCGTGCTAGATGCGTGCGTAATTTATCCATCTCTCCTGCGGGATTTATTAATCCACTT
AGGACTAACAGGGCTGTATCAGCCTAAGTGGACGGCCATCATCGAGGATGAATGGCAACGCAATCTACTGCTGAATAGAC
CTGATTTAACCCCGGAGCAAATCTCGCGGACACGCGAGCTGATGAATCAGGCTATTCCTGATGCGATGATTACTGGGTTT
GAAGCGCTCATTCCAGGCATCACGCTCCCTGATCCTGATGATCAGCATGTGGTTGCAGCCGCCATTCGCAGTAACGCTGA
AATCATCGTTACGTTTAACCTGAAAGATTTTCCCCATCCCGAGATCGATAACTTTGGCATTGAAGCGCTACACCCGGATG
ATTTCATCACAGACCTGCTCGATTTGAATCAGGCGCTTGTGTTATCCGCAGTAAGCAGGCAGCGTCACAGCATGAGAAAA
CCGCCTAAATCGGTTGATGAGTATCTTGATGCGTTACTCCGGCAGAGCTTACCGCAAACAGTAAAAGAATTGAGTAAATT
CCACGCCCTGATTTAG

Antitoxin

Protein

• Length: 154 a.a.
• Molecular weight: 16913.27 Da
• Isoelectric Point: 6.2221

>AT75598 WP_038917616.1 NZ_CP020872:431649-432110 [Dickeya fangzhongdai]
MTNSILDSLSLPAPGEIEAAVRGQRELAAYLSTKMETQKISIQDADNNTRQVELPTSALTLLMSILGELAVGNAVQVVPV
HAELTTQEAANILNVSRPHMVKLLEDGKLPFHKTGRHRRVLFADLMDYKHQRDNESSRAMQALADHSQELGLY

Nucleotide

• Length: 462 bp

>AT75598 NZ_CP020872:431649-432110 [Dickeya fangzhongdai]
ATGACAAACTCAATTCTTGATAGCCTGAGCCTTCCGGCACCAGGGGAGATTGAGGCGGCTGTTCGTGGACAAAGGGAGCT
TGCTGCCTATCTCTCCACAAAGATGGAAACACAAAAAATCTCGATTCAGGACGCAGATAACAACACTCGTCAGGTCGAAT
TACCGACGTCGGCACTGACATTATTGATGTCGATTTTAGGCGAGCTGGCTGTGGGTAACGCGGTTCAGGTTGTTCCTGTT
CATGCTGAATTAACGACACAGGAGGCAGCCAACATTCTGAATGTTTCACGCCCTCACATGGTGAAGCTTCTGGAGGATGG
GAAACTACCTTTTCATAAAACCGGTCGCCACCGCCGTGTGCTTTTCGCTGACCTGATGGACTATAAACACCAGAGGGATA
ATGAAAGCAGTAGAGCAATGCAGGCGTTGGCAGATCATAGCCAAGAGCTTGGATTGTACTAA