Detailed information of TA system TA075597

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 18837..19359
• Replicon: plasmid pXF-De_Donno
• Accession: NZ_CP020871
• Organism: Xylella fastidiosa subsp. pauca strain De Donno

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: B9J09_RS11995
• Protein ID: WP_004085905.1
• Coordinates: 18837..19124 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A060HDF9
• Locus tag: B9J09_RS12000
• Protein ID: WP_004085758.1
• Coordinates: 19117..19359 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B9J09_RS11985	13890..18005	+	4116	WP_046417500.1	DUF1738 domain-containing protein	-
B9J09_RS11990	18192..18749	-	558	WP_046417502.1	recombinase family protein	-
B9J09_RS11995	18837..19124	-	288	WP_004085905.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
B9J09_RS12000	19117..19359	-	243	WP_004085758.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
B9J09_RS12005	19777..20097	+	321	WP_046417511.1	type II toxin-antitoxin system RelE/ParE family toxin	-
B9J09_RS12010	20227..20553	+	327	WP_004091396.1	DNA-binding protein	-
B9J09_RS12510	20928..21101	-	174	WP_155641416.1	hypothetical protein	-
B9J09_RS12015	21103..21375	-	273	WP_046417513.1	type II toxin-antitoxin system RelE/ParE family toxin	-
B9J09_RS12020	21347..21844	+	498	Protein_23	Tn3 family transposase	-
B9J09_RS12025	21875..22456	-	582	Protein_24	conjugal transfer protein TrbN	-
B9J09_RS12030	22462..23859	-	1398	WP_046417516.1	P-type conjugative transfer protein TrbL	-
B9J09_RS12515	23865..24179	-	315	WP_155561293.1	EexN family lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..35273	35273
	-	inside	IScluster/Tn	-	-	18192..21844	3652

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-90)

Antitoxin

• Antitoxin ParD (1-78)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11159.64 Da
• Isoelectric Point: 5.9570

>T75597 WP_004085905.1 NZ_CP020871:c19124-18837 [Xylella fastidiosa subsp. pauca]
MAKSYRLTPLAEADLEEIWFYTFRHWSIEQADSYHRSLVAVFEGLAAGTKQGCPSVLPDFNKYLCGSHVVYFMDYADHLD
IIRILHQRQDTERYL

Nucleotide

• Length: 288 bp

>T75597 NZ_CP020871:c19124-18837 [Xylella fastidiosa subsp. pauca]
ATGGCTAAATCCTATCGACTGACACCGCTGGCCGAGGCCGATCTTGAAGAAATCTGGTTCTACACATTCAGGCACTGGTC
GATAGAGCAGGCCGACAGCTACCACCGCAGCCTAGTTGCAGTTTTCGAGGGGCTGGCAGCAGGTACAAAGCAAGGCTGCC
CTTCTGTTTTGCCGGACTTCAATAAATACCTATGTGGTTCGCATGTAGTGTATTTCATGGATTACGCAGATCACTTGGAT
ATTATCCGCATCCTGCATCAGCGGCAGGATACCGAGCGGTACCTATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8774.77 Da
• Isoelectric Point: 6.9881

>AT75597 WP_004085758.1 NZ_CP020871:c19359-19117 [Xylella fastidiosa subsp. pauca]
MSRNTSVALGPHFTSFIDAQVQGGRYGTASDVVRAGLRLLEEHETKVKALQDALNVGHQSGEPRPFDSEVFLSRMHTQHG

Nucleotide

• Length: 243 bp

>AT75597 NZ_CP020871:c19359-19117 [Xylella fastidiosa subsp. pauca]
ATGAGTCGAAACACATCCGTTGCCCTCGGGCCGCACTTCACCAGCTTTATTGATGCCCAAGTCCAGGGCGGTCGCTACGG
TACCGCCAGCGATGTTGTCAGGGCAGGTCTGCGGCTGCTGGAGGAACACGAAACAAAGGTGAAGGCACTGCAAGATGCGC
TGAATGTTGGCCACCAATCCGGCGAGCCGCGCCCGTTCGACAGCGAGGTGTTTTTGAGCCGGATGCACACGCAGCATGGC
TAA

Structures

Antitoxin

• AlphaFold DB: A0A060HDF9