Detailed information of TA system TA075594

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 1383019..1383611
• Replicon: chromosome
• Accession: NZ_CP020870
• Organism: Xylella fastidiosa subsp. pauca strain De Donno

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: B9J09_RS06835
• Protein ID: WP_046419679.1
• Coordinates: 1383019..1383315 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: B9J09_RS06840
• Protein ID: WP_004085592.1
• Coordinates: 1383318..1383611 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B9J09_RS06805	1378557..1379048	-	492	WP_171817079.1	glutathione peroxidase	-
B9J09_RS12410	1379501..1379662	-	162	WP_010894100.1	hypothetical protein	-
B9J09_RS06815	1379881..1380756	+	876	WP_023906641.1	ABC transporter ATP-binding protein	-
B9J09_RS06820	1380753..1381730	+	978	WP_046419683.1	hypothetical protein	-
B9J09_RS06825	1381972..1382418	+	447	WP_031336674.1	hypothetical protein	-
B9J09_RS06830	1382608..1382827	-	220	Protein_1247	tail fiber protein	-
B9J09_RS06835	1383019..1383315	+	297	WP_046419679.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
B9J09_RS06840	1383318..1383611	+	294	WP_004085592.1	putative addiction module antidote protein	Antitoxin
B9J09_RS06845	1383667..1384020	-	354	WP_046419676.1	hypothetical protein	-
B9J09_RS06850	1384017..1384397	-	381	Protein_1251	hypothetical protein	-
B9J09_RS06855	1384935..1385951	+	1017	WP_060870179.1	peptidase M4 family protein	-
B9J09_RS06860	1385938..1386312	+	375	WP_060870180.1	hypothetical protein	-
B9J09_RS06865	1386336..1386608	+	273	WP_060871504.1	helix-turn-helix domain-containing protein	-
B9J09_RS06870	1386580..1387146	+	567	WP_081089935.1	SocA family protein	-
B9J09_RS06875	1387146..1387373	+	228	WP_046419974.1	hypothetical protein	-
B9J09_RS06880	1387374..1388537	-	1164	WP_046419971.1	phage tail protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1358970..1459130	100160

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Uncharacterized protein HI1420 (3-93)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11141.65 Da
• Isoelectric Point: 9.8173

>T75594 WP_046419679.1 NZ_CP020870:1383019-1383315 [Xylella fastidiosa subsp. pauca]
MIELKQTDTFRKWREKLKDARARSAIASRLDRLAFGHVGDAEPVGKGVSELRINYGPGYRVYFQQRGDTIYLLLCGGDKG
SQARDIKTALHLSEQWSE

Nucleotide

• Length: 297 bp

>T75594 NZ_CP020870:1383019-1383315 [Xylella fastidiosa subsp. pauca]
ATGATTGAATTGAAGCAGACTGACACCTTCCGCAAGTGGCGGGAGAAACTCAAGGATGCGCGCGCCCGCTCGGCCATCGC
CTCACGCCTCGACCGCTTGGCGTTCGGCCATGTCGGCGACGCCGAGCCAGTAGGGAAAGGTGTCAGCGAGCTTCGCATCA
ACTACGGCCCCGGTTACCGGGTGTATTTCCAGCAGCGTGGCGACACGATCTACTTGCTACTTTGCGGCGGTGACAAAGGA
TCACAAGCGCGTGACATCAAGACTGCGCTGCACCTCTCTGAACAATGGAGCGAATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10246.59 Da
• Isoelectric Point: 5.6232

>AT75594 WP_004085592.1 NZ_CP020870:1383318-1383611 [Xylella fastidiosa subsp. pauca]
MTEKLTSYDPAEDLTTDQAIADFMAAAFGTNDPAYVAHALGVVARAKGMTQIASQTGLSREQLYRSFSAEGNPTLRTTLA
VMKALGIELSAKPSGVH

Nucleotide

• Length: 294 bp

>AT75594 NZ_CP020870:1383318-1383611 [Xylella fastidiosa subsp. pauca]
ATGACCGAGAAACTGACGAGCTACGATCCGGCCGAGGACTTGACCACTGACCAGGCCATCGCCGATTTCATGGCAGCCGC
GTTCGGGACGAACGATCCTGCCTACGTTGCCCACGCGCTGGGCGTCGTTGCCCGCGCCAAGGGCATGACGCAGATCGCCA
GCCAGACAGGGCTATCGCGCGAACAGCTCTACCGCTCGTTCAGTGCCGAGGGCAACCCGACGCTGCGCACGACATTAGCC
GTGATGAAGGCGCTAGGGATCGAGCTCTCTGCGAAACCGTCTGGTGTTCACTGA