Detailed information of TA system TA075593

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 1296694..1297286
• Replicon: chromosome
• Accession: NZ_CP020870
• Organism: Xylella fastidiosa subsp. pauca strain De Donno

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A060HCK8
• Locus tag: B9J09_RS06310
• Protein ID: WP_004085590.1
• Coordinates: 1296694..1296990 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: B9J09_RS06315
• Protein ID: WP_004085592.1
• Coordinates: 1296993..1297286 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B9J09_RS06285	1292925..1293494	+	570	WP_046420067.1	SocA family protein	-
B9J09_RS06290	1293494..1293721	+	228	WP_046419974.1	hypothetical protein	-
B9J09_RS06295	1293722..1294885	-	1164	WP_046419971.1	phage tail protein	-
B9J09_RS06300	1294889..1295449	-	561	WP_046419969.1	DUF2612 domain-containing protein	-
B9J09_RS06305	1295446..1296591	-	1146	WP_046419967.1	baseplate J/gp47 family protein	-
B9J09_RS06310	1296694..1296990	+	297	WP_004085590.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
B9J09_RS06315	1296993..1297286	+	294	WP_004085592.1	putative addiction module antidote protein	Antitoxin
B9J09_RS06320	1297342..1297695	-	354	WP_046419963.1	hypothetical protein	-
B9J09_RS06325	1297692..1298333	-	642	WP_046419962.1	hypothetical protein	-
B9J09_RS06330	1298330..1299160	-	831	WP_046419672.1	hypothetical protein	-
B9J09_RS06335	1299157..1299474	-	318	WP_046419669.1	hypothetical protein	-
B9J09_RS06340	1299474..1300244	-	771	WP_046419667.1	hypothetical protein	-
B9J09_RS06345	1300652..1300909	-	258	WP_046419904.1	Blp family class II bacteriocin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1286889..1337775	50886

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-94)

Antitoxin

• Uncharacterized protein HI1420 (3-93)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11169.70 Da
• Isoelectric Point: 10.0610

>T75593 WP_004085590.1 NZ_CP020870:1296694-1296990 [Xylella fastidiosa subsp. pauca]
MIELKQTDTFRKWREKLKDARARSAIASRLDRLAFGHVGDAEPVGKGVSELRINYGPGYRVYFQRRGDTIYLLLCGGDKG
SQARDIKTALHLSEQWSE

Nucleotide

• Length: 297 bp

>T75593 NZ_CP020870:1296694-1296990 [Xylella fastidiosa subsp. pauca]
ATGATTGAATTGAAGCAGACTGACACCTTCCGCAAGTGGCGGGAGAAACTCAAGGATGCGCGCGCCCGCTCGGCCATCGC
CTCGCGCCTCGACCGCTTGGCGTTCGGCCATGTCGGCGACGCGGAGCCAGTAGGGAAAGGTGTCAGCGAGCTTCGCATCA
ACTACGGCCCCGGTTACCGGGTGTATTTCCAGCGGCGTGGCGACACGATCTACTTGCTGCTTTGCGGCGGTGACAAAGGA
TCACAAGCGCGCGACATCAAGACTGCGCTGCACCTGTCTGAACAATGGAGCGAATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10246.59 Da
• Isoelectric Point: 5.6232

>AT75593 WP_004085592.1 NZ_CP020870:1296993-1297286 [Xylella fastidiosa subsp. pauca]
MTEKLTSYDPAEDLTTDQAIADFMAAAFGTNDPAYVAHALGVVARAKGMTQIASQTGLSREQLYRSFSAEGNPTLRTTLA
VMKALGIELSAKPSGVH

Nucleotide

• Length: 294 bp

>AT75593 NZ_CP020870:1296993-1297286 [Xylella fastidiosa subsp. pauca]
ATGACCGAGAAACTGACGAGCTACGATCCAGCCGAGGACTTGACCACTGACCAGGCCATCGCCGATTTCATGGCAGCTGC
GTTCGGGACGAACGATCCTGCCTACGTTGCCCACGCGCTGGGCGTCGTTGCCCGCGCCAAGGGCATGACGCAGATCGCCA
GCCAAACAGGGCTATCGCGCGAACAGCTCTACCGCTCGTTCAGTGCCGAGGGCAACCCAACGCTGCGCACGACATTGGCC
GTGATGAAGGCGCTAGGGATCGAGCTGTCTGCGAAACCGTCGGGTGTTCACTGA

Structures

Toxin

• AlphaFold DB: A0A060HCK8