Detailed information of TA system TA075589

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 4779666..4780231
• Replicon: chromosome
• Accession: NZ_CP020866
• Organism: Paenibacillus sp. Cedars

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: B9D94_RS23670
• Protein ID: WP_148248112.1
• Coordinates: 4779666..4780007 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: W4AXS2
• Locus tag: B9D94_RS23675
• Protein ID: WP_006208406.1
• Coordinates: 4780001..4780231 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B9D94_RS23645	4775298..4776795	+	1498	Protein_4370	PucR family transcriptional regulator ligand-binding domain-containing protein	-
B9D94_RS23650	4776930..4777244	-	315	WP_148248109.1	hypothetical protein	-
B9D94_RS23655	4777421..4777774	-	354	WP_076153060.1	hypothetical protein	-
B9D94_RS23660	4777784..4778758	-	975	WP_148248110.1	WXG100 family type VII secretion target	-
B9D94_RS23665	4779040..4779555	-	516	WP_148248111.1	DUF4241 domain-containing protein	-
B9D94_RS23670	4779666..4780007	-	342	WP_148248112.1	endoribonuclease MazF	Toxin
B9D94_RS23675	4780001..4780231	-	231	WP_006208406.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
B9D94_RS23680	4780466..4781218	-	753	WP_006208405.1	superoxide dismutase family protein	-
B9D94_RS23685	4781414..4781854	-	441	WP_006208404.1	hypothetical protein	-
B9D94_RS23690	4781887..4782543	-	657	Protein_4379	hypothetical protein	-
B9D94_RS23695	4782747..4782971	+	225	WP_006208402.1	hypothetical protein	-
B9D94_RS23700	4783227..4784591	+	1365	WP_148248113.1	guanine deaminase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-111)

Antitoxin

• SpoVT-AbrB domain (5-46)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12729.83 Da
• Isoelectric Point: 7.8522

>T75589 WP_148248112.1 NZ_CP020866:c4780007-4779666 [Paenibacillus sp. Cedars]
MVKEQYIPDRGDLVWIQFNPQSGHEQSGKRPALVLSPVTYNGKVGLVLFCPVTSKAKGYPFEVVIPQDLPIEGVILSDQI
KSLDWQSRQAKFICRVPKETLNEVLSKVDLLIH

Nucleotide

• Length: 342 bp

>T75589 NZ_CP020866:c4780007-4779666 [Paenibacillus sp. Cedars]
ATGGTAAAAGAACAATACATTCCTGATCGTGGCGACTTGGTATGGATTCAGTTTAATCCTCAATCTGGTCATGAGCAATC
GGGGAAACGGCCAGCTCTTGTACTCTCACCTGTAACGTATAACGGTAAGGTCGGTTTAGTCTTATTTTGTCCGGTTACTT
CCAAGGCTAAAGGCTACCCCTTTGAGGTTGTTATTCCTCAGGATTTACCGATAGAAGGGGTTATTCTTTCTGATCAAATC
AAGAGTCTCGATTGGCAATCTCGCCAAGCCAAGTTTATTTGCAGAGTTCCTAAGGAGACCTTGAATGAGGTTTTGTCTAA
GGTAGATTTATTGATTCATTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8451.48 Da
• Isoelectric Point: 4.3077

>AT75589 WP_006208406.1 NZ_CP020866:c4780231-4780001 [Paenibacillus sp. Cedars]
MSQVQKWGNSLGIRIPKALAMEIGLEEGSEIDLDVEDGHLVIKPRTNTLEDLLSKITPDNIHSEVQTGEPQGRESW

Nucleotide

• Length: 231 bp

>AT75589 NZ_CP020866:c4780231-4780001 [Paenibacillus sp. Cedars]
ATGTCGCAAGTTCAAAAATGGGGAAACAGTTTAGGCATTCGCATCCCAAAGGCACTGGCCATGGAGATAGGACTTGAGGA
GGGATCAGAAATCGATCTTGACGTTGAAGATGGTCATCTCGTCATCAAACCACGGACCAACACACTGGAAGATCTACTTT
CGAAAATAACGCCTGATAATATCCATTCCGAGGTTCAAACGGGTGAGCCGCAAGGACGGGAGTCATGGTAA

Structures

Antitoxin

• AlphaFold DB: W4AXS2