Detailed information of TA system TA075588

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 718187..718813
• Replicon: chromosome
• Accession: NZ_CP020866
• Organism: Paenibacillus sp. Cedars

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: W4ARZ2
• Locus tag: B9D94_RS05005
• Protein ID: WP_006212388.1
• Coordinates: 718463..718813 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: B9D94_RS05000
• Protein ID: WP_006212389.1
• Coordinates: 718187..718459 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B9D94_RS04980	713261..713761	+	501	WP_006212393.1	winged helix-turn-helix domain-containing protein	-
B9D94_RS04985	713820..715148	+	1329	WP_148246548.1	MFS transporter	-
B9D94_RS04990	715381..716493	+	1113	WP_148246549.1	outer membrane lipoprotein carrier protein LolA	-
B9D94_RS30880	716518..716667	+	150	WP_160164035.1	hypothetical protein	-
B9D94_RS04995	716764..717951	+	1188	WP_148246550.1	alanine racemase	-
B9D94_RS05000	718187..718459	+	273	WP_006212389.1	ribbon-helix-helix protein, CopG family	Antitoxin
B9D94_RS05005	718463..718813	+	351	WP_006212388.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
B9D94_RS05010	719274..721487	+	2214	WP_063480494.1	RNA-binding transcriptional accessory protein	-
B9D94_RS05015	721677..722120	-	444	WP_148246551.1	GNAT family N-acetyltransferase	-
B9D94_RS05020	722182..722742	-	561	WP_040681006.1	CGNR zinc finger domain-containing protein	-
B9D94_RS05025	722889..723359	+	471	WP_148246552.1	DinB family protein	-
B9D94_RS05030	723467..723598	-	132	WP_006212382.1	cortex morphogenetic protein CmpA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-43)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12751.64 Da
• Isoelectric Point: 4.8265

>T75588 WP_006212388.1 NZ_CP020866:718463-718813 [Paenibacillus sp. Cedars]
VIVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAASHGFDRDSVILLEQI
RTIDKQRLTDKITHLDDEMMSKVDDSLQISLGLIDF

Nucleotide

• Length: 351 bp

>T75588 NZ_CP020866:718463-718813 [Paenibacillus sp. Cedars]
GTGATCGTTAAACGGGGCGACGTATTTTTTGCGGATTTATCACCCGTTGTCGGTTCGGAGCAGGGCGGCGTAAGGCCGGT
TCTTGTGATCCAGAACGATATCGGCAACCGGTTTAGTCCGACTGTGATTGTTGCAGCCATTACGGCACAGATTCAAAAAG
CCAAGCTGCCGACTCACGTCGAAATCGACGCGGCGTCGCATGGATTTGATCGGGATTCCGTCATTTTGCTAGAGCAGATC
CGGACGATTGATAAGCAGCGGCTAACTGACAAAATCACCCATCTTGATGATGAAATGATGAGCAAGGTGGATGATTCCCT
GCAAATAAGTCTGGGCTTGATTGATTTTTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10500.04 Da
• Isoelectric Point: 6.4708

>AT75588 WP_006212389.1 NZ_CP020866:718187-718459 [Paenibacillus sp. Cedars]
MQNTKRIMISLPDHLLQEVDGIAQLENSNRSELIRQAMKLYLNERKKRYIRESMQRGYMEMAKINLTMASEAFHAEEDAD
NTLDRLVSGV

Nucleotide

• Length: 273 bp

>AT75588 NZ_CP020866:718187-718459 [Paenibacillus sp. Cedars]
ATGCAGAACACCAAACGAATCATGATCAGTTTGCCGGATCATCTTTTGCAGGAAGTGGACGGGATCGCTCAGCTGGAAAA
TTCCAACCGCAGTGAGCTTATTAGGCAGGCCATGAAGTTGTACCTGAATGAACGGAAGAAACGTTACATTCGTGAATCCA
TGCAGCGAGGTTATATGGAAATGGCCAAAATTAACCTGACCATGGCTTCTGAGGCGTTTCACGCGGAGGAAGATGCAGAC
AACACTCTAGACCGCTTAGTAAGCGGGGTGTAG

Structures

Toxin

• AlphaFold DB: A0A1S2EZN8