Detailed information of TA system TA075579

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 232761..233869
• Replicon: plasmid unnamed1
• Accession: NZ_CP020859
• Organism: Ligilactobacillus salivarius strain ZLS006

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: R3JIN1
• Locus tag: B7R82_RS10610
• Protein ID: WP_000233000.1
• Coordinates: 233000..233869 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: B7R82_RS10605
• Protein ID: WP_000205227.1
• Coordinates: 232761..232985 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7R82_RS10575	228156..228515	+	360	WP_003703258.1	hypothetical protein	-
B7R82_RS10580	228531..229343	+	813	WP_003706971.1	SPFH domain-containing protein	-
B7R82_RS10585	229397..229732	+	336	WP_167370873.1	hypothetical protein	-
B7R82_RS10590	230341..230637	+	297	WP_002360685.1	hypothetical protein	-
B7R82_RS10595	230638..231054	+	417	WP_087449133.1	recombinase	-
B7R82_RS10600	231056..232618	+	1563	WP_000136908.1	recombinase family protein	-
B7R82_RS10605	232761..232985	+	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
B7R82_RS10610	233000..233869	+	870	WP_000233000.1	nucleotidyltransferase domain-containing protein	Toxin
B7R82_RS10615	233850..234584	+	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
B7R82_RS10620	234617..235480	+	864	WP_002294505.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
B7R82_RS10625	235524..236023	+	500	Protein_230	adenine phosphoribosyltransferase	-
B7R82_RS10635	236109..237281	+	1173	WP_000195429.1	IS256-like element IS256 family transposase	-
B7R82_RS10645	237411..238850	-	1440	WP_001028144.1	aminoglycoside O-phosphotransferase APH(2'')-Ia	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32926.57 Da
• Isoelectric Point: 4.8781

>T75579 WP_000233000.1 NZ_CP020859:233000-233869 [Ligilactobacillus salivarius]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T75579 NZ_CP020859:233000-233869 [Ligilactobacillus salivarius]
ATGGTAGGTAATGTTATTCAAATAGTAACAGAGAAATTATCATCTTTGCCTTTTATAGAAGGCATCGTATTAGGGGGCTC
ACGTGCAAGAGGCACCCATACAGAAAATTCTGATATAGATATCGGAATCTATTACAATTCAGACTCATTTGACCTGACAG
CGATTAATCAAATTGCTACAGAATTGGATGATAAAAATAGAAACAACCTTGTTGTGCCTCCCGGAGCGTGGGGTGATTGG
ATTAATGGCGGCGGATGGTTATTTATAAATGGGTATCATGTTGACTTGATTTTACGTGATATAAAACGGGTAGAACAAAT
AATCAAAGATACGGAGCAAGGAATTGTTACTGCCAATTATCAGACCGGGCATCCCCATGGTTATATAAGTGCAATGTATC
GTGGAGAATTAGCGATTAGTAAAATACTATATGCTAAGAACGAAAGCTTATGCGAATTAAAAAAGCAGGCAGAAATTTAC
CCTACTGCTCTAAAGAAAAGCTTGATGAACTTTTTTATATTTGAAGCTGAATTCTCTTTAATGTTTGTAAAAGCAAATGC
GGGGGTAGAGGATAAATATTATATTGCAGGCCATGTTTTTCGTATTATTTCATGCTTAAATCAAGTACTATTTGCATGTA
ATAATGCTTATTGCATTAACGAAAAGAAAGCTATAAAACTGCTTGAAACTTTTGAATATAAACCTGAAAAATATGCCGAA
AGAGTTAATCATATTTTTGAAGTACTCGGTCTTTCTCTTTTTGAATGCTACGATATGACCGAGAAACTTTATAAAGAAGT
GAATGAAATTGTATCGGAGATAAATAACTTTTTAAACGAGGAGAGTTCAGATGAAAGAAAACAAATATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT75579 WP_000205227.1 NZ_CP020859:232761-232985 [Ligilactobacillus salivarius]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT75579 NZ_CP020859:232761-232985 [Ligilactobacillus salivarius]
ATGACTACGGCAGAAATGATTAAAGAACTGTGTGAGCAAATGAATATAAGTGTTTCCGAACTTGCTAGACGTATTGGCCA
GACTCCACAGAATTTCAATAAAAAATTACAACGAGAAACAGTAACCTTGGATGAGTTGAAGGCCATTGCTGATGTGCTAG
GTGTCAAGTTTGTGCAGGCATTTATTTTACCAGATGGAGAAGAAATAAAAATATCTAACGAATAA

Structures

Toxin

• AlphaFold DB: R3JIN1