Detailed information of TA system TA075576

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 21198..21841
• Replicon: plasmid pKPN528-3
• Accession: NZ_CP020856
• Organism: Klebsiella pneumoniae strain KPN528

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: B8F96_RS29750
• Protein ID: WP_001044770.1
• Coordinates: 21198..21614 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: B8F96_RS29755
• Protein ID: WP_001261282.1
• Coordinates: 21611..21841 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B8F96_RS29725	17783..18115	+	333	WP_001393253.1	WbuC family cupin fold metalloprotein	-
B8F96_RS29730	18162..19037	-	876	WP_000239590.1	class A extended-spectrum beta-lactamase CTX-M-15	-
B8F96_RS29735	19293..19667	-	375	Protein_22	transposase	-
B8F96_RS29740	19683..20380	-	698	Protein_23	IS1 family transposase	-
B8F96_RS29745	20414..21124	-	711	WP_004152334.1	ATP-binding protein	-
B8F96_RS29750	21198..21614	-	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
B8F96_RS29755	21611..21841	-	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
B8F96_RS31025	21798..22259	+	462	WP_072202616.1	hypothetical protein	-
B8F96_RS29765	22403..22753	+	351	WP_000493378.1	hypothetical protein	-
B8F96_RS29770	22804..23547	+	744	WP_000129823.1	hypothetical protein	-
B8F96_RS29775	23544..24320	+	777	WP_000015958.1	site-specific integrase	-
B8F96_RS29780	24378..24635	-	258	WP_000764642.1	hypothetical protein	-
B8F96_RS31030	24764..24868	-	105	WP_032409716.1	hypothetical protein	-
B8F96_RS29785	25403..26269	+	867	WP_004118283.1	replication initiation protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaSHV-187 / aac(3)-IId / blaCTX-M-15 / sul2 / aph(3'')-Ib / aph(6)-Id / sul1 / qacE / cmlA1 / ant(3'')-Ia / ere(A) / ARR-3	-	1..76158	76158

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T75576 WP_001044770.1 NZ_CP020856:c21614-21198 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T75576 NZ_CP020856:c21614-21198 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT75576 WP_001261282.1 NZ_CP020856:c21841-21611 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT75576 NZ_CP020856:c21841-21611 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3