Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 142978..143714 Replicon plasmid pKPN528-2
Accession NZ_CP020855
Organism Klebsiella pneumoniae strain KPN528

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag B8F96_RS29150 Protein ID WP_003026803.1
Coordinates 142978..143460 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag B8F96_RS29155 Protein ID WP_003026799.1
Coordinates 143448..143714 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B8F96_RS29135 138615..139577 + 963 WP_004152113.1 M48 family metalloprotease -
B8F96_RS29140 139665..140919 + 1255 Protein_144 IS3 family transposase -
B8F96_RS29145 141420..142766 - 1347 WP_020314316.1 ISNCY family transposase -
B8F96_RS29150 142978..143460 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
B8F96_RS29155 143448..143714 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
B8F96_RS29160 143890..144144 - 255 WP_004152108.1 hypothetical protein -
B8F96_RS29165 144220..144477 - 258 WP_004152107.1 hypothetical protein -
B8F96_RS29170 144526..144729 - 204 WP_004152106.1 hemolysin expression modulator Hha -
B8F96_RS29175 144763..145131 - 369 WP_004152105.1 hypothetical protein -
B8F96_RS29180 145175..145669 - 495 WP_004152104.1 DNA-binding protein -
B8F96_RS29185 145700..146275 - 576 WP_004152103.1 hypothetical protein -
B8F96_RS29190 146263..146532 - 270 WP_004152102.1 hypothetical protein -
B8F96_RS29195 146890..147240 + 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
B8F96_RS29200 147290..147652 + 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid catA1 - 1..221428 221428


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T75575 WP_003026803.1 NZ_CP020855:c143460-142978 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T75575 NZ_CP020855:c143460-142978 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT75575 WP_003026799.1 NZ_CP020855:c143714-143448 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT75575 NZ_CP020855:c143714-143448 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References