Detailed information of TA system TA075572

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/ElaA-DUF1778
• Location: 45751..46502
• Replicon: plasmid pKPN528-1
• Accession: NZ_CP020854
• Organism: Klebsiella pneumoniae strain KPN528

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: H6U1U8
• Locus tag: B8F96_RS26885
• Protein ID: WP_014386536.1
• Coordinates: 45751..46233 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A071LPN3
• Locus tag: B8F96_RS26890
• Protein ID: WP_004902250.1
• Coordinates: 46224..46502 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B8F96_RS26855	42150..42797	-	648	WP_014386537.1	EcsC family protein	-
B8F96_RS26860	42824..43579	-	756	WP_004902235.1	DUF2971 domain-containing protein	-
B8F96_RS26865	43680..44072	-	393	WP_032442757.1	hypothetical protein	-
B8F96_RS26870	44177..44716	-	540	WP_004902239.1	hypothetical protein	-
B8F96_RS26885	45751..46233	-	483	WP_014386536.1	GNAT family N-acetyltransferase	Toxin
B8F96_RS26890	46224..46502	-	279	WP_004902250.1	DUF1778 domain-containing protein	Antitoxin
B8F96_RS26895	46621..46833	-	213	WP_004902255.1	hypothetical protein	-
B8F96_RS26900	46941..47282	-	342	WP_004902257.1	hypothetical protein	-
B8F96_RS26910	48112..48570	-	459	WP_014386535.1	hypothetical protein	-
B8F96_RS26915	49223..49678	-	456	WP_001166628.1	Hg(II)-responsive transcriptional regulator	-
B8F96_RS26920	49750..50115	+	366	WP_004200999.1	mercuric ion transporter MerT	-
B8F96_RS26925	50131..50406	+	276	WP_000732275.1	mercury resistance system periplasmic binding protein MerP	-
B8F96_RS26930	50434..50859	+	426	WP_000522996.1	organomercurial transporter MerC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaNDM-1 / aph(3')-VI / mph(E) / msr(E) / armA / sul1 / qacE / aadA2 / dfrA12 / dfrA14 / blaOXA-1 / aac(6')-Ib-cr / qnrB1	-	1..292471	292471

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-86)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17662.45 Da
• Isoelectric Point: 8.9130

>T75572 WP_014386536.1 NZ_CP020854:c46233-45751 [Klebsiella pneumoniae]
MGMRAPESLTAEHNIAEFCCQEPALNEWLKKKALRNHSTGISRVYVVCAENTNRVIGYYCLSSGSVHRNTVPGAYRRNAP
DVIPVIVLGRLAIDQAWAGNGLGAALLKDAIYRTQNIAFQVGVRALAVHALNEEVKCFYTRFGFEPSIVNTLTLLFPIKV

Nucleotide

• Length: 483 bp

>T75572 NZ_CP020854:c46233-45751 [Klebsiella pneumoniae]
ATGGGAATGAGAGCGCCTGAATCCCTGACGGCTGAACATAACATCGCTGAGTTCTGCTGCCAGGAGCCTGCCCTTAATGA
GTGGTTAAAGAAGAAGGCTCTCAGAAACCACAGTACCGGCATTTCCCGTGTCTATGTCGTCTGCGCAGAAAACACTAACC
GGGTCATCGGTTATTATTGTTTATCTTCGGGTAGCGTCCACCGTAACACAGTGCCTGGGGCCTATAGACGCAACGCGCCT
GATGTCATTCCAGTCATTGTTTTGGGACGGCTTGCCATCGACCAGGCCTGGGCCGGGAATGGGCTTGGTGCCGCACTTCT
TAAGGATGCTATATACCGTACGCAAAACATAGCCTTTCAGGTCGGGGTGAGAGCACTGGCCGTGCATGCGCTGAATGAAG
AAGTTAAATGTTTTTACACCCGTTTTGGCTTTGAGCCTTCCATAGTCAACACGCTGACATTGTTATTCCCGATAAAAGTC
TGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10704.23 Da
• Isoelectric Point: 4.5271

>AT75572 WP_004902250.1 NZ_CP020854:c46502-46224 [Klebsiella pneumoniae]
MKPDVREAPINIRAKTSQRDLIDMAASLVSKSRTDFMLEVACREAQDILLDQRLFLLNDNQFEAFIEELDAPITPERQAR
IDNLMNRKSPWE

Nucleotide

• Length: 279 bp

>AT75572 NZ_CP020854:c46502-46224 [Klebsiella pneumoniae]
ATGAAACCAGACGTTCGTGAAGCTCCGATTAATATCCGGGCCAAAACATCACAGCGTGACCTGATCGACATGGCCGCAAG
CCTGGTCTCAAAATCACGGACTGATTTTATGCTTGAGGTTGCCTGCAGGGAGGCACAGGACATTCTGCTTGATCAACGCC
TGTTCCTCCTCAATGACAATCAGTTTGAGGCGTTTATTGAAGAGCTTGACGCCCCAATAACGCCGGAGCGCCAGGCGCGA
ATCGATAACCTGATGAACCGCAAATCACCATGGGAATGA

Structures

Toxin

• AlphaFold DB: A0A2A5MBI1

Antitoxin

• AlphaFold DB: A0A071LPN3