Detailed information of TA system TA075554

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 11066..11709
• Replicon: plasmid pKPN1481-3
• Accession: NZ_CP020852
• Organism: Klebsiella variicola strain KPN1481

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: B8O08_RS30765
• Protein ID: WP_049141430.1
• Coordinates: 11293..11709 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: B8O08_RS30760
• Protein ID: WP_049141429.1
• Coordinates: 11066..11296 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B8O08_RS30705	6132..6410	-	279	WP_020316685.1	helix-turn-helix domain-containing protein	-
B8O08_RS30710	6400..6719	-	320	Protein_11	type II toxin-antitoxin system RelE/ParE family toxin	-
B8O08_RS30715	6800..7024	-	225	WP_004152719.1	hypothetical protein	-
B8O08_RS30720	7035..7247	-	213	WP_025714932.1	hypothetical protein	-
B8O08_RS30725	7307..7663	-	357	WP_013023814.1	hypothetical protein	-
B8O08_RS30740	8300..8650	-	351	WP_004152758.1	hypothetical protein	-
B8O08_RS30745	8647..8919	-	273	WP_032445785.1	hypothetical protein	-
B8O08_RS30750	9358..9516	-	159	WP_014386184.1	type I toxin-antitoxin system Hok family toxin	-
B8O08_RS30755	9588..10661	+	1074	WP_013609528.1	IS481-like element ISKpn28 family transposase	-
B8O08_RS30760	11066..11296	+	231	WP_049141429.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
B8O08_RS30765	11293..11709	+	417	WP_049141430.1	type II toxin-antitoxin system VapC family toxin	Toxin
B8O08_RS30770	11892..13157	-	1266	WP_080901827.1	hypothetical protein	-
B8O08_RS30775	13452..14665	-	1214	Protein_22	IS91 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..20380	20380
	-	flank	IS/Tn	-	-	9588..10661	1073
	-	flank	IS/Tn	-	-	16390..16995	605

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 14897.28 Da
• Isoelectric Point: 7.2449

>T75554 WP_049141430.1 NZ_CP020852:11293-11709 [Klebsiella variicola]
VIKTYMLDTNICSFIMREQPEAVIKRLEQAVLRNHRIVVSAITYAEMRFGAIGKKASPRHGLLVEAFCARLDAILAWDRA
AVDATTEIKAALTAAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWAS

Nucleotide

• Length: 417 bp

>T75554 NZ_CP020852:11293-11709 [Klebsiella variicola]
GTGATTAAAACCTATATGCTGGACACTAACATTTGCTCGTTCATCATGCGTGAGCAGCCGGAAGCTGTCATTAAACGACT
GGAACAGGCAGTGCTGCGCAATCACCGCATTGTGGTTTCGGCCATCACTTATGCTGAGATGCGTTTCGGTGCAATCGGAA
AGAAGGCATCCCCTCGCCACGGCCTGCTTGTCGAAGCTTTCTGCGCCCGTCTCGATGCAATCCTCGCCTGGGACCGCGCA
GCGGTGGACGCAACCACGGAGATTAAGGCTGCGTTGACTGCAGCCGGTACACCTATCGGTCCCAACGACACAGCGATTGC
CGGGCATGCTATCGCCGCTGGCGCCGTGCTGGTGACGAACAATGTGCGTGAGTTTGAACGTGTGCCCGGCCTGGTCCTGG
AAGACTGGGCTAGCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8667.69 Da
• Isoelectric Point: 4.3193

>AT75554 WP_049141429.1 NZ_CP020852:11066-11296 [Klebsiella variicola]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFTQLENADPDFMAERGDVVSDEGRFDL

Nucleotide

• Length: 231 bp

>AT75554 NZ_CP020852:11066-11296 [Klebsiella variicola]
ATGAGAACCGTATCGATATTCAAGAATGGCAACAATCGCGCCATCCGTCTGCCCCGTGACCTGGATTTTGAAGGAGTGAG
TGAACTGGAAATCGTCCGTGAAGGTGACAGCATCATCCTTCGTCCCGTTCGCCCAACATGGGGTTCGTTTACTCAGCTCG
AAAATGCTGATCCGGACTTTATGGCCGAGCGTGGTGATGTTGTCAGCGATGAAGGACGGTTTGACCTGTGA