Detailed information of TA system TA075553

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 96999..97642
• Replicon: plasmid pKPN1481-2
• Accession: NZ_CP020851
• Organism: Klebsiella variicola strain KPN1481

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: B8O08_RS30210
• Protein ID: WP_049141430.1
• Coordinates: 96999..97415 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: B8O08_RS30215
• Protein ID: WP_049141429.1
• Coordinates: 97412..97642 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B8O08_RS30190	92587..93015	+	429	WP_001554382.1	GFA family protein	-
B8O08_RS30200	94043..95256	+	1214	Protein_91	IS91 family transposase	-
B8O08_RS30205	95551..96816	+	1266	WP_080901827.1	hypothetical protein	-
B8O08_RS30210	96999..97415	-	417	WP_049141430.1	type II toxin-antitoxin system VapC family toxin	Toxin
B8O08_RS30215	97412..97642	-	231	WP_049141429.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
B8O08_RS30220	98091..98249	+	159	WP_014386184.1	type I toxin-antitoxin system Hok family toxin	-
B8O08_RS30225	98688..98960	+	273	WP_032445785.1	hypothetical protein	-
B8O08_RS30230	98957..99307	+	351	WP_004152758.1	hypothetical protein	-
B8O08_RS30245	99944..100300	+	357	WP_013023814.1	hypothetical protein	-
B8O08_RS30250	100361..100573	+	213	WP_025714932.1	hypothetical protein	-
B8O08_RS30255	100584..100808	+	225	WP_004152719.1	hypothetical protein	-
B8O08_RS30260	100889..101209	+	321	WP_004152720.1	type II toxin-antitoxin system RelE/ParE family toxin	-
B8O08_RS30265	101199..101477	+	279	WP_020316685.1	helix-turn-helix domain-containing protein	-
B8O08_RS30270	101478..101891	+	414	WP_065954386.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3'')-Ib / aph(6)-Id	-	1..161100	161100
	-	flank	IS/Tn	-	-	91697..92302	605

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 14897.28 Da
• Isoelectric Point: 7.2449

>T75553 WP_049141430.1 NZ_CP020851:c97415-96999 [Klebsiella variicola]
VIKTYMLDTNICSFIMREQPEAVIKRLEQAVLRNHRIVVSAITYAEMRFGAIGKKASPRHGLLVEAFCARLDAILAWDRA
AVDATTEIKAALTAAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWAS

Nucleotide

• Length: 417 bp

>T75553 NZ_CP020851:c97415-96999 [Klebsiella variicola]
GTGATTAAAACCTATATGCTGGACACTAACATTTGCTCGTTCATCATGCGTGAGCAGCCGGAAGCTGTCATTAAACGACT
GGAACAGGCAGTGCTGCGCAATCACCGCATTGTGGTTTCGGCCATCACTTATGCTGAGATGCGTTTCGGTGCAATCGGAA
AGAAGGCATCCCCTCGCCACGGCCTGCTTGTCGAAGCTTTCTGCGCCCGTCTCGATGCAATCCTCGCCTGGGACCGCGCA
GCGGTGGACGCAACCACGGAGATTAAGGCTGCGTTGACTGCAGCCGGTACACCTATCGGTCCCAACGACACAGCGATTGC
CGGGCATGCTATCGCCGCTGGCGCCGTGCTGGTGACGAACAATGTGCGTGAGTTTGAACGTGTGCCCGGCCTGGTCCTGG
AAGACTGGGCTAGCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8667.69 Da
• Isoelectric Point: 4.3193

>AT75553 WP_049141429.1 NZ_CP020851:c97642-97412 [Klebsiella variicola]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFTQLENADPDFMAERGDVVSDEGRFDL

Nucleotide

• Length: 231 bp

>AT75553 NZ_CP020851:c97642-97412 [Klebsiella variicola]
ATGAGAACCGTATCGATATTCAAGAATGGCAACAATCGCGCCATCCGTCTGCCCCGTGACCTGGATTTTGAAGGAGTGAG
TGAACTGGAAATCGTCCGTGAAGGTGACAGCATCATCCTTCGTCCCGTTCGCCCAACATGGGGTTCGTTTACTCAGCTCG
AAAATGCTGATCCGGACTTTATGGCCGAGCGTGGTGATGTTGTCAGCGATGAAGGACGGTTTGACCTGTGA