Detailed information of TA system TA075551

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 330372..331282
• Replicon: plasmid pKPN1481-1
• Accession: NZ_CP020848
• Organism: Klebsiella variicola strain KPN1481

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A663AYG0
• Locus tag: B8O08_RS29420
• Protein ID: WP_004026354.1
• Coordinates: 330812..331282 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A2J4RLY9
• Locus tag: B8O08_RS29415
• Protein ID: WP_004181895.1
• Coordinates: 330372..330815 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B8O08_RS29395	327005..328492	+	1488	WP_004178082.1	group II intron reverse transcriptase/maturase	-
B8O08_RS29405	328578..329000	+	423	WP_004181893.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
B8O08_RS29410	329000..330271	+	1272	WP_004181894.1	translesion error-prone DNA polymerase V subunit UmuC	-
B8O08_RS29415	330372..330815	+	444	WP_004181895.1	DUF2384 domain-containing protein	Antitoxin
B8O08_RS29420	330812..331282	+	471	WP_004026354.1	RES family NAD+ phosphorylase	Toxin
B8O08_RS29425	331392..331652	-	261	WP_004026352.1	hypothetical protein	-
B8O08_RS29430	332352..333721	+	1370	WP_085949440.1	IS3-like element ISKpn8 family transposase	-
B8O08_RS29435	333740..334852	-	1113	WP_014386573.1	IS481-like element ISKpn28 family transposase	-
B8O08_RS29440	334924..335082	+	159	WP_004181898.1	type I toxin-antitoxin system Hok family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(6')-Ib / ant(3'')-Ia / blaOXA-9 / blaTEM-1A / qnrB1 / blaNDM-1 / aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15	htpB	1..347748	347748

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17515.79 Da
• Isoelectric Point: 4.6155

>T75551 WP_004026354.1 NZ_CP020848:330812-331282 [Klebsiella variicola]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T75551 NZ_CP020848:330812-331282 [Klebsiella variicola]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACCCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATACAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16536.87 Da
• Isoelectric Point: 10.3013

>AT75551 WP_004181895.1 NZ_CP020848:330372-330815 [Klebsiella variicola]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT75551 NZ_CP020848:330372-330815 [Klebsiella variicola]
ATGAAAACTTTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
TGTAGCGCTCCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGTGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTTATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACGGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Toxin

• AlphaFold DB: A0A663AYG0

Antitoxin

• AlphaFold DB: A0A2J4RLY9