Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) stbDE/ParE-DnaT
Location 192375..192897 Replicon plasmid pKPN1481-1
Accession NZ_CP020848
Organism Klebsiella variicola strain KPN1481

Toxin (Protein)


Gene name stbE Uniprot ID A0A2J4R0S6
Locus tag B8O08_RS28660 Protein ID WP_004181778.1
Coordinates 192613..192897 (+) Length 95 a.a.

Antitoxin (Protein)


Gene name stbD Uniprot ID A0A5Q9LM53
Locus tag B8O08_RS28655 Protein ID WP_025368601.1
Coordinates 192375..192623 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B8O08_RS28635 187575..188396 - 822 WP_004026535.1 hypothetical protein -
B8O08_RS28640 188458..188811 - 354 WP_004181774.1 hypothetical protein -
B8O08_RS28645 188956..189951 - 996 WP_046654939.1 hypothetical protein -
B8O08_RS28650 190285..192084 - 1800 WP_042935572.1 ATP-dependent helicase -
B8O08_RS28655 192375..192623 + 249 WP_025368601.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
B8O08_RS28660 192613..192897 + 285 WP_004181778.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
B8O08_RS28670 193127..194353 + 1227 WP_042935575.1 transposase -
B8O08_RS28680 194639..195064 - 426 WP_040120489.1 IS200/IS605 family transposase -
B8O08_RS28685 195101..196327 + 1227 WP_025368604.1 transposase -
B8O08_RS31210 196393..197519 - 1127 Protein_212 conjugal transfer protein TraG N-terminal domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(6')-Ib / ant(3'')-Ia / blaOXA-9 / blaTEM-1A / qnrB1 / blaNDM-1 / aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 htpB 1..347748 347748


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-84)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10970.69 Da        Isoelectric Point: 10.6516

>T75550 WP_004181778.1 NZ_CP020848:192613-192897 [Klebsiella variicola]
MTYKLAFNESALKEWKKLGHTIREQFKKKLAERLLNPRVPASQLHGRKDQYKIKLRGAGYRLVYSVNDDVVTVTVIGVGK
RENDDIYNLTKHRN

Download         Length: 285 bp

>T75550 NZ_CP020848:192613-192897 [Klebsiella variicola]
ATGACCTATAAGCTGGCCTTTAATGAATCAGCTCTGAAAGAATGGAAAAAATTGGGCCATACCATTCGGGAACAATTCAA
AAAGAAGCTCGCTGAGCGCCTGTTGAACCCACGGGTGCCCGCATCGCAACTCCATGGACGCAAAGACCAGTACAAAATAA
AGCTCCGTGGCGCAGGCTACCGACTGGTTTACAGTGTCAACGATGATGTTGTAACGGTCACCGTAATTGGCGTCGGTAAA
CGTGAGAATGATGATATTTACAATTTGACCAAACACAGAAATTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8906.12 Da        Isoelectric Point: 3.8206

>AT75550 WP_025368601.1 NZ_CP020848:192375-192623 [Klebsiella variicola]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMELLEDQELGHIADERIGEAVIEVSLD
DL

Download         Length: 249 bp

>AT75550 NZ_CP020848:192375-192623 [Klebsiella variicola]
ATGGCATTTCAGGTACTAACCACTACTGCAGCAAGCATCACAGAGCTTAAACGTGATCCTATGGGCACCTTCAATGCAGG
AGAAGGTGCCCCGGTAGCCATACTGAATCGCAACGAACCCGCTTTTTACTGTGTACCGCCTGCTCTGTATGCTCAACTGA
TGGAGCTTCTTGAAGATCAGGAGCTGGGTCATATTGCTGATGAGCGAATTGGTGAGGCGGTAATCGAGGTCAGTCTAGAT
GACCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4R0S6

Antitoxin

Source ID Structure
AlphaFold DB A0A5Q9LM53

References