Detailed information of TA system TA075549

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 155282..156035
• Replicon: plasmid pKPN1481-1
• Accession: NZ_CP020848
• Organism: Klebsiella variicola strain KPN1481

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: R8WN26
• Locus tag: B8O08_RS28430
• Protein ID: WP_016154457.1
• Coordinates: 155658..156035 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: B8O08_RS28425
• Protein ID: WP_162838704.1
• Coordinates: 155282..155608 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B8O08_RS28365	150341..150607	+	267	WP_009652486.1	hypothetical protein	-
B8O08_RS28370	150620..150931	+	312	WP_009652410.1	hypothetical protein	-
B8O08_RS28375	150978..151448	+	471	WP_009652431.1	hypothetical protein	-
B8O08_RS28380	151497..152009	+	513	WP_009652395.1	DUF4234 domain-containing protein	-
B8O08_RS28385	152092..152313	+	222	WP_009652443.1	hypothetical protein	-
B8O08_RS28390	152369..152602	+	234	WP_009652474.1	DUF905 domain-containing protein	-
B8O08_RS28395	152706..153524	+	819	WP_009652424.1	DUF945 domain-containing protein	-
B8O08_RS28400	153524..153778	+	255	WP_009652519.1	hypothetical protein	-
B8O08_RS28405	153807..154268	+	462	WP_045890390.1	antirestriction protein	-
B8O08_RS28410	154279..154497	+	219	WP_009652503.1	hypothetical protein	-
B8O08_RS28415	154523..154990	+	468	WP_086893240.1	DNA repair protein RadC	-
B8O08_RS28420	155004..155225	+	222	WP_009652392.1	DUF987 domain-containing protein	-
B8O08_RS28425	155282..155608	+	327	WP_162838704.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
B8O08_RS28430	155658..156035	+	378	WP_016154457.1	TA system toxin CbtA family protein	Toxin
B8O08_RS28435	156032..156523	+	492	WP_009652438.1	hypothetical protein	-
B8O08_RS28440	156553..156756	+	204	WP_009652505.1	DUF957 domain-containing protein	-
B8O08_RS28445	156837..157682	+	846	Protein_168	DUF4942 domain-containing protein	-
B8O08_RS32020	157694..157921	+	228	WP_164996479.1	hypothetical protein	-
B8O08_RS28450	157954..158912	+	959	Protein_170	hypothetical protein	-
B8O08_RS28455	158915..159388	+	474	WP_009652494.1	ribbon-helix-helix protein, CopG family	-
B8O08_RS28460	159708..160346	+	639	WP_009652437.1	VWA domain-containing protein	-
B8O08_RS28465	160371..161012	+	642	WP_009652489.1	TerD family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(6')-Ib / ant(3'')-Ia / blaOXA-9 / blaTEM-1A / qnrB1 / blaNDM-1 / aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15	htpB	1..347748	347748

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (4-103)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14168.19 Da
• Isoelectric Point: 9.5224

>T75549 WP_016154457.1 NZ_CP020848:155658-156035 [Klebsiella variicola]
MQTQPVPPKREVSPRPSPVAIWQRLLSHLLDRHYGLTLNDTPFGKDGVIQEHIDAGISLCDAVNFIVEKYDLVRTDRRGF
NAETQSPLLTSIDILRARKATGLMTRHSYRAVTDITTGKYREVQP

Nucleotide

• Length: 378 bp

>T75549 NZ_CP020848:155658-156035 [Klebsiella variicola]
ATGCAAACACAACCTGTTCCTCCGAAACGGGAGGTTTCGCCACGCCCGTCACCCGTGGCGATCTGGCAGCGCCTCCTGAG
TCACCTGCTGGACCGTCACTACGGTCTGACGCTCAACGACACGCCATTTGGCAAAGATGGTGTGATCCAAGAGCATATCG
ATGCCGGTATCTCACTGTGCGACGCGGTGAATTTCATCGTGGAGAAATACGACCTGGTACGCACTGATCGTCGCGGTTTT
AACGCGGAAACTCAGTCGCCGCTTCTCACCAGTATCGATATTCTCCGCGCCCGCAAAGCCACCGGGCTGATGACCCGTCA
TAGCTACAGGGCCGTTACCGACATCACCACCGGCAAATACCGTGAGGTGCAACCATGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12079.85 Da
• Isoelectric Point: 6.6283

>AT75549 WP_162838704.1 NZ_CP020848:155282-155608 [Klebsiella variicola]
IAAPWWGLKRDITPCFGARLVQEGNRLHYLNDRASITGTFSDADLRHLDQAFPLLLKQLELMLLSGELNPRHQHCVTLYA
KGLICEADSLGSHGYVYLAIYPTPATTE

Nucleotide

• Length: 327 bp

>AT75549 NZ_CP020848:155282-155608 [Klebsiella variicola]
ATCGCCGCCCCTTGGTGGGGGCTTAAACGCGATATCACCCCCTGTTTTGGCGCACGTCTGGTGCAGGAGGGCAACCGGCT
GCATTACCTGAACGACCGCGCCAGCATCACCGGCACCTTCAGCGACGCGGATTTACGCCATCTCGACCAGGCATTCCCGC
TACTGCTGAAGCAACTGGAGCTGATGCTGCTTTCCGGTGAGCTGAATCCCCGTCATCAGCACTGCGTCACGCTGTATGCA
AAAGGGCTGATATGCGAGGCCGACAGCCTCGGCTCGCACGGGTACGTCTATCTCGCAATTTACCCGACACCTGCAACAAC
AGAATAA

Structures

Toxin

• AlphaFold DB: R8WN26