Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3725180..3725799 Replicon chromosome
Accession NZ_CP020847
Organism Klebsiella variicola strain KPN1481

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag B8O08_RS18395 Protein ID WP_002892050.1
Coordinates 3725180..3725398 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A1F2MBN7
Locus tag B8O08_RS18400 Protein ID WP_008805436.1
Coordinates 3725425..3725799 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B8O08_RS18365 3721223..3721486 + 264 WP_008805431.1 type B 50S ribosomal protein L31 -
B8O08_RS18370 3721486..3721626 + 141 WP_086892962.1 type B 50S ribosomal protein L36 -
B8O08_RS18375 3721623..3722321 - 699 WP_012968815.1 GNAT family N-acetyltransferase -
B8O08_RS18380 3722422..3723879 + 1458 WP_016160392.1 PLP-dependent aminotransferase family protein -
B8O08_RS18385 3723848..3724318 - 471 WP_008805434.1 YlaC family protein -
B8O08_RS31720 3724355..3724483 + 129 Protein_3475 hypothetical protein -
B8O08_RS18390 3724455..3725021 - 567 WP_032438710.1 maltose O-acetyltransferase -
B8O08_RS18395 3725180..3725398 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
B8O08_RS18400 3725425..3725799 - 375 WP_008805436.1 Hha toxicity modulator TomB Antitoxin
B8O08_RS18410 3726282..3729428 - 3147 WP_008805437.1 multidrug efflux RND transporter permease subunit -
B8O08_RS18415 3729451..3730644 - 1194 WP_008805438.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T75547 WP_002892050.1 NZ_CP020847:c3725398-3725180 [Klebsiella variicola]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T75547 NZ_CP020847:c3725398-3725180 [Klebsiella variicola]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAACTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACCATGAATAAGCTGTACGATAAGATCCCGACCTCAGTCTGGAAGTTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14384.06 Da        Isoelectric Point: 4.8989

>AT75547 WP_008805436.1 NZ_CP020847:c3725799-3725425 [Klebsiella variicola]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYTEDNKLVAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT75547 NZ_CP020847:c3725799-3725425 [Klebsiella variicola]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACTTCGGCTGTCAATCTTCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCGCTTAATTATAAAATTAAGTACACTGAGGATAATAAACTGGTCGCTCAGGTTGACGAATATCTG
GACGATACTTTTACCTTGTTCAGTAACTACGGCATTAACTCAACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTCTCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2MBN7

References