75544

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2493944..2494569 Replicon chromosome
Accession NZ_CP020847
Organism Klebsiella variicola strain KPN1481

Toxin (Protein)


Gene name vapC Uniprot ID A0A1F2M041
Locus tag B8O08_RS12430 Protein ID WP_008807903.1
Coordinates 2494186..2494569 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag B8O08_RS12425 Protein ID WP_004150355.1
Coordinates 2493944..2494186 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B8O08_RS12400 2489934..2490533 + 600 WP_008807908.1 glucose-1-phosphatase -
B8O08_RS12405 2490527..2491387 + 861 WP_008807907.1 virulence factor BrkB family protein -
B8O08_RS12410 2491384..2491821 + 438 WP_012543288.1 D-tyrosyl-tRNA(Tyr) deacylase -
B8O08_RS12415 2491866..2492807 + 942 WP_012543287.1 fatty acid biosynthesis protein FabY -
B8O08_RS12420 2492822..2493739 - 918 WP_032733990.1 alpha/beta hydrolase -
B8O08_RS12425 2493944..2494186 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
B8O08_RS12430 2494186..2494569 + 384 WP_008807903.1 type II toxin-antitoxin system VapC family toxin Toxin
B8O08_RS12435 2494743..2495672 - 930 WP_055323209.1 formate dehydrogenase accessory protein FdhE -
B8O08_RS12440 2495669..2496304 - 636 WP_008807902.1 formate dehydrogenase cytochrome b556 subunit -
B8O08_RS12445 2496301..2497203 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T75544 WP_008807903.1 NZ_CP020847:2494186-2494569 [Klebsiella variicola]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYELHPE

Download         Length: 384 bp

>T75544 NZ_CP020847:2494186-2494569 [Klebsiella variicola]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGTACGCGAATGGCGCTAAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTGATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAACTACACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT75544 WP_004150355.1 NZ_CP020847:2493944-2494186 [Klebsiella variicola]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT75544 NZ_CP020847:2493944-2494186 [Klebsiella variicola]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2M041

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References