Detailed information of TA system TA075543

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 2076957..2077543
• Replicon: chromosome
• Accession: NZ_CP020847
• Organism: Klebsiella variicola strain KPN1481

Toxin (Protein)

• Gene name: doc
• Uniprot ID: W8VD46
• Locus tag: B8O08_RS10445
• Protein ID: WP_002920800.1
• Coordinates: 2076957..2077325 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: B8O08_RS10450
• Protein ID: WP_086892792.1
• Coordinates: 2077322..2077543 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B8O08_RS10425	2072441..2073511	-	1071	WP_008806977.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-
B8O08_RS10430	2073513..2074358	-	846	WP_008806976.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
B8O08_RS10435	2074355..2075242	-	888	WP_012967120.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
B8O08_RS10440	2075349..2076665	-	1317	WP_086892791.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
B8O08_RS10445	2076957..2077325	-	369	WP_002920800.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
B8O08_RS10450	2077322..2077543	-	222	WP_086892792.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
B8O08_RS10455	2077707..2078420	-	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
B8O08_RS10460	2078422..2079189	-	768	WP_032731362.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
B8O08_RS10465	2079186..2080463	-	1278	WP_008806971.1	branched chain amino acid ABC transporter permease LivM	-
B8O08_RS10470	2080460..2081386	-	927	WP_012540389.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2071704..2092187	20483

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (13-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13552.92 Da
• Isoelectric Point: 8.6410

>T75543 WP_002920800.1 NZ_CP020847:c2077325-2076957 [Klebsiella variicola]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T75543 NZ_CP020847:c2077325-2076957 [Klebsiella variicola]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGACCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCGGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCGGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGCGGATGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8181.18 Da
• Isoelectric Point: 4.7769

>AT75543 WP_086892792.1 NZ_CP020847:c2077543-2077322 [Klebsiella variicola]
MRTVNYSEARQNLADVLESAVTGIPVTITRRGHKSAVIISSEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT75543 NZ_CP020847:c2077543-2077322 [Klebsiella variicola]
ATGAGAACGGTTAACTATAGCGAAGCCCGACAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTATACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCGGTCATCATTAGTTCAGAAGAATTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATTATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A0H3GUD1