Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 591229..591819 Replicon chromosome
Accession NZ_CP020847
Organism Klebsiella variicola strain KPN1481

Toxin (Protein)


Gene name pemK Uniprot ID A0A1F2LYA2
Locus tag B8O08_RS03010 Protein ID WP_008804165.1
Coordinates 591229..591561 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A1F2LZQ3
Locus tag B8O08_RS03015 Protein ID WP_012541132.1
Coordinates 591562..591819 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B8O08_RS02985 587267..587512 + 246 WP_008804171.1 signal transduction protein PmrD -
B8O08_RS02990 587643..589097 + 1455 WP_008804170.1 AMP nucleosidase -
B8O08_RS03000 589535..590971 + 1437 WP_016161429.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
B8O08_RS03010 591229..591561 - 333 WP_008804165.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
B8O08_RS03015 591562..591819 - 258 WP_012541132.1 antitoxin Antitoxin
B8O08_RS03025 592347..593447 + 1101 WP_086892631.1 phosphotransferase -
B8O08_RS03030 593444..594415 - 972 WP_064165897.1 sensor domain-containing diguanylate cyclase -
B8O08_RS03035 594621..595457 - 837 WP_008804155.1 alpha/beta hydrolase -
B8O08_RS03040 595636..596211 - 576 WP_064165898.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11867.71 Da        Isoelectric Point: 10.1839

>T75540 WP_008804165.1 NZ_CP020847:c591561-591229 [Klebsiella variicola]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDAVVNEVLARLDAILS

Download         Length: 333 bp

>T75540 NZ_CP020847:c591561-591229 [Klebsiella variicola]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTCCTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACCCGGCTACCGGTCGTGGTTCCCGTCACCAGCGGTGGAAACTTTGCACGTA
CAGCAGGCTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGAACTATC
GATATGGCGGCCCGGAACGGCAAGCGTCTGGAACGCATACCAGACGCCGTTGTCAATGAAGTGCTTGCCAGGCTGGATGC
GATTCTCAGTTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9420.91 Da        Isoelectric Point: 4.4915

>AT75540 WP_012541132.1 NZ_CP020847:c591819-591562 [Klebsiella variicola]
MYKTRLKKVGGSIMLAVPPAVLKALELSTDSEVGMTIDNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT75540 NZ_CP020847:c591819-591562 [Klebsiella variicola]
ATGTACAAAACTCGCCTGAAAAAGGTTGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAGCGCTGGAGCT
GTCGACAGACAGCGAAGTGGGTATGACTATAGATAATGGCTGCCTGATTATTGAACCGCAGAAACGGCCCCATTATTCGC
TTGAGGAACTGTTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATTCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2LYA2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2LZQ3

References