Detailed information of TA system TA075531

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 44181..44824
• Replicon: plasmid pKPN1482-2
• Accession: NZ_CP020843
• Organism: Klebsiella pneumoniae strain KPN1482

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: A0A7D6UII7
• Locus tag: B8O09_RS29860
• Protein ID: WP_032425563.1
• Coordinates: 44408..44824 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: R4IT22
• Locus tag: B8O09_RS29855
• Protein ID: WP_016338373.1
• Coordinates: 44181..44411 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B8O09_RS31790	39331..39633	+	303	WP_071571079.1	hypothetical protein	-
B8O09_RS29815	40169..40963	-	795	WP_004197635.1	site-specific integrase	-
B8O09_RS29820	41161..42177	-	1017	WP_017899884.1	hypothetical protein	-
B8O09_RS29825	42188..42502	-	315	WP_053389906.1	hypothetical protein	-
B8O09_RS29830	42529..42924	-	396	WP_017899885.1	hypothetical protein	-
B8O09_RS29835	43093..43398	-	306	WP_013023785.1	type II toxin-antitoxin system toxin CcdB	-
B8O09_RS29840	43400..43618	-	219	WP_001568025.1	type II toxin-antitoxin system antitoxin CcdA	-
B8O09_RS29855	44181..44411	+	231	WP_016338373.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
B8O09_RS29860	44408..44824	+	417	WP_032425563.1	type II toxin-antitoxin system VapC family toxin	Toxin
B8O09_RS29865	44865..45743	-	879	WP_009309916.1	restriction endonuclease	-
B8O09_RS29870	46405..47546	+	1142	Protein_64	HlyD family secretion protein	-
B8O09_RS31120	47580..47710	+	131	Protein_65	IS5/IS1182 family transposase	-
B8O09_RS29875	47734..48129	-	396	Protein_66	IS6-like element IS26 family transposase	-
B8O09_RS29880	48193..48897	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
B8O09_RS29890	48946..49260	-	315	Protein_68	DUF2913 family protein	-
B8O09_RS29895	49314..49595	-	282	WP_086893463.1	cytoplasmic protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..97202	97202
	-	flank	IS/Tn	-	-	48193..48897	704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-50)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15080.56 Da
• Isoelectric Point: 8.5403

>T75531 WP_032425563.1 NZ_CP020843:44408-44824 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T75531 NZ_CP020843:44408-44824 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGCCACGCCATCGCGGCCGGCGCCATCCTGGTGACGAATAATACAAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8751.77 Da
• Isoelectric Point: 4.3533

>AT75531 WP_016338373.1 NZ_CP020843:44181-44411 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWSSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT75531 NZ_CP020843:44181-44411 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGAGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: A0A7D6UII7

Antitoxin

• AlphaFold DB: A0A5Q9LN61