Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 43093..43618 Replicon plasmid pKPN1482-2
Accession NZ_CP020843
Organism Klebsiella pneumoniae strain KPN1482

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag B8O09_RS29835 Protein ID WP_013023785.1
Coordinates 43093..43398 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag B8O09_RS29840 Protein ID WP_001568025.1
Coordinates 43400..43618 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B8O09_RS29805 38708..39334 + 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -
B8O09_RS31790 39331..39633 + 303 WP_071571079.1 hypothetical protein -
B8O09_RS29815 40169..40963 - 795 WP_004197635.1 site-specific integrase -
B8O09_RS29820 41161..42177 - 1017 WP_017899884.1 hypothetical protein -
B8O09_RS29825 42188..42502 - 315 WP_053389906.1 hypothetical protein -
B8O09_RS29830 42529..42924 - 396 WP_017899885.1 hypothetical protein -
B8O09_RS29835 43093..43398 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
B8O09_RS29840 43400..43618 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
B8O09_RS29855 44181..44411 + 231 WP_016338373.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
B8O09_RS29860 44408..44824 + 417 WP_032425563.1 type II toxin-antitoxin system VapC family toxin -
B8O09_RS29865 44865..45743 - 879 WP_009309916.1 restriction endonuclease -
B8O09_RS29870 46405..47546 + 1142 Protein_64 HlyD family secretion protein -
B8O09_RS31120 47580..47710 + 131 Protein_65 IS5/IS1182 family transposase -
B8O09_RS29875 47734..48129 - 396 Protein_66 IS6-like element IS26 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..97202 97202
- flank IS/Tn - - 48193..48897 704


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T75530 WP_013023785.1 NZ_CP020843:c43398-43093 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T75530 NZ_CP020843:c43398-43093 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT75530 WP_001568025.1 NZ_CP020843:c43618-43400 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT75530 NZ_CP020843:c43618-43400 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References