Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 65742..66478 Replicon plasmid pKPN1482-1
Accession NZ_CP020842
Organism Klebsiella pneumoniae strain KPN1482

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag B8O09_RS28710 Protein ID WP_003026803.1
Coordinates 65996..66478 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag B8O09_RS28705 Protein ID WP_003026799.1
Coordinates 65742..66008 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B8O09_RS28660 61804..62166 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
B8O09_RS28665 62216..62566 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
B8O09_RS28670 62924..63193 + 270 WP_004152102.1 hypothetical protein -
B8O09_RS28675 63181..63756 + 576 WP_004152103.1 hypothetical protein -
B8O09_RS28680 63787..64281 + 495 WP_004152104.1 DNA-binding protein -
B8O09_RS28685 64325..64693 + 369 WP_004152105.1 hypothetical protein -
B8O09_RS28690 64727..64930 + 204 WP_004152106.1 hemolysin expression modulator Hha -
B8O09_RS28695 64979..65236 + 258 WP_004152107.1 hypothetical protein -
B8O09_RS28700 65312..65566 + 255 WP_004152108.1 hypothetical protein -
B8O09_RS28705 65742..66008 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
B8O09_RS28710 65996..66478 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
B8O09_RS28715 66679..68082 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
B8O09_RS28720 68111..68743 - 633 WP_001567369.1 hypothetical protein -
B8O09_RS28725 68969..70315 + 1347 WP_077253394.1 ISNCY family transposase -
B8O09_RS28730 70364..70759 + 396 WP_015632387.1 helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid rmtF / ARR-3 / qnrB1 - 1..180210 180210


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T75529 WP_003026803.1 NZ_CP020842:65996-66478 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T75529 NZ_CP020842:65996-66478 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT75529 WP_003026799.1 NZ_CP020842:65742-66008 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT75529 NZ_CP020842:65742-66008 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References