Detailed information of TA system TA075524

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 3815395..3816098
• Replicon: chromosome
• Accession: NZ_CP020841
• Organism: Klebsiella pneumoniae strain KPN1482

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: B8O09_RS19205
• Protein ID: WP_065803899.1
• Coordinates: 3815757..3816098 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A377WBK0
• Locus tag: B8O09_RS19200
• Protein ID: WP_053274373.1
• Coordinates: 3815395..3815736 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B8O09_RS19160	3810937..3811641	+	705	WP_065803893.1	WYL domain-containing protein	-
B8O09_RS19165	3811641..3812210	+	570	WP_004929046.1	hypothetical protein	-
B8O09_RS19170	3812247..3812699	+	453	WP_065803894.1	hypothetical protein	-
B8O09_RS19175	3812696..3813145	+	450	WP_071571046.1	hypothetical protein	-
B8O09_RS19180	3813217..3813438	+	222	WP_065803896.1	DUF905 domain-containing protein	-
B8O09_RS19185	3813537..3814358	+	822	WP_065803897.1	DUF945 domain-containing protein	-
B8O09_RS19190	3814389..3814829	+	441	WP_039567646.1	antirestriction protein	-
B8O09_RS19195	3814842..3815384	+	543	WP_065803898.1	DNA repair protein RadC	-
B8O09_RS19200	3815395..3815736	+	342	WP_053274373.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
B8O09_RS19205	3815757..3816098	+	342	WP_065803899.1	TA system toxin CbtA family protein	Toxin
B8O09_RS19215	3816353..3817360	+	1008	WP_004108748.1	restriction endonuclease	-
B8O09_RS31000	3817728..3818839	+	1112	Protein_3688	IS3 family transposase	-
B8O09_RS31005	3818856..3819044	+	189	Protein_3689	transposase	-
B8O09_RS19250	3819243..3820127	-	885	WP_004192285.1	RES domain-containing protein	-
B8O09_RS19255	3820138..3820810	-	673	Protein_3691	DUF4400 domain-containing protein	-
B8O09_RS19260	3820810..3821076	-	267	WP_004178415.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3791178..3827641	36463

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-109)

Antitoxin

• Antitoxin CbeA (13-112)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12756.74 Da
• Isoelectric Point: 9.0089

>T75524 WP_065803899.1 NZ_CP020841:3815757-3816098 [Klebsiella pneumoniae]
MKTLPATTPQAVTLCLSPVAVWQMLLARLLEQHYGLTLNDTPFSDETVIHEHIDAGISLADAVNFLVEKYGLVRIDRRGF
NWQEQSPYLRAVDILRARQATGLLKRNRISAAR

Nucleotide

• Length: 342 bp

>T75524 NZ_CP020841:3815757-3816098 [Klebsiella pneumoniae]
ATGAAAACTCTACCTGCAACAACTCCGCAGGCGGTGACGCTATGTCTGTCGCCCGTGGCAGTGTGGCAAATGCTGCTTGC
ACGCCTGCTGGAACAACATTATGGCCTGACACTCAACGACACACCATTCAGTGATGAAACGGTAATTCATGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATTTTCTGGTGGAAAAGTACGGGCTGGTTCGTATTGATCGCAGGGGATTC
AACTGGCAAGAACAGTCCCCATATCTGCGGGCGGTCGATATTCTGCGGGCGCGACAGGCAACTGGTTTATTGAAAAGAAA
TCGTATTAGCGCGGCACGGTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12773.41 Da
• Isoelectric Point: 6.6253

>AT75524 WP_053274373.1 NZ_CP020841:3815395-3815736 [Klebsiella pneumoniae]
MKSTDSENDSFLKWGLNRNVTPCFGARLVQEGNRLHYLADRASITGKFSEAECLKLDVAFPHFISQMESMLTTGEMNPRH
AHCVTLYHNGFTCEADTLGSYGYVYIAVYPTQR

Nucleotide

• Length: 342 bp

>AT75524 NZ_CP020841:3815395-3815736 [Klebsiella pneumoniae]
ATGAAATCTACTGACTCAGAGAACGATTCATTTCTCAAGTGGGGTCTGAATCGTAATGTGACACCTTGCTTTGGCGCTCG
TCTGGTACAGGAGGGAAACCGGCTGCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTCAGCGAAGCAGAATGTC
TTAAGCTGGATGTGGCATTCCCACATTTTATCAGCCAGATGGAGTCGATGTTGACTACTGGTGAAATGAATCCTCGCCAT
GCCCACTGTGTCACCCTGTACCACAATGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTACGGCTACGTATACATCGC
CGTTTACCCCACTCAACGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A377WBK0