Detailed information of TA system TA075507

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 204522..205258
• Replicon: plasmid pBK13043-1
• Accession: NZ_CP020838
• Organism: Klebsiella pneumoniae strain BK13043

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: B9G35_RS29410
• Protein ID: WP_003026803.1
• Coordinates: 204776..205258 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: B9G35_RS29405
• Protein ID: WP_003026799.1
• Coordinates: 204522..204788 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B9G35_RS29360	200584..200946	-	363	WP_004152100.1	arsenite efflux transporter metallochaperone ArsD	-
B9G35_RS29365	200996..201346	-	351	WP_004152101.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
B9G35_RS29370	201704..201973	+	270	WP_004152102.1	hypothetical protein	-
B9G35_RS29375	201961..202536	+	576	WP_004152103.1	hypothetical protein	-
B9G35_RS29380	202567..203061	+	495	WP_004152104.1	DNA-binding protein	-
B9G35_RS29385	203105..203473	+	369	WP_004152105.1	hypothetical protein	-
B9G35_RS29390	203507..203710	+	204	WP_004152106.1	hemolysin expression modulator Hha	-
B9G35_RS29395	203759..204016	+	258	WP_004152107.1	hypothetical protein	-
B9G35_RS29400	204092..204346	+	255	WP_004152108.1	hypothetical protein	-
B9G35_RS29405	204522..204788	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
B9G35_RS29410	204776..205258	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
B9G35_RS29415	205470..206816	+	1347	WP_020314316.1	ISNCY family transposase	-
B9G35_RS29420	207317..208571	-	1255	Protein_217	IS3 family transposase	-
B9G35_RS29425	208659..209621	-	963	WP_004152113.1	M48 family metalloprotease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-Ia / mph(A) / sul1 / qacE / aadA2 / dfrA12 / catA1	-	1..232540	232540

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T75507 WP_003026803.1 NZ_CP020838:204776-205258 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T75507 NZ_CP020838:204776-205258 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT75507 WP_003026799.1 NZ_CP020838:204522-204788 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT75507 NZ_CP020838:204522-204788 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66