Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 21349..21871 Replicon plasmid pCFSAN051542
Accession NZ_CP020836
Organism Escherichia coli strain CFSAN051542

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag BCD20_RS25660 Protein ID WP_000220560.1
Coordinates 21590..21871 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag BCD20_RS25655 Protein ID WP_000121743.1
Coordinates 21349..21600 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BCD20_RS25625 16675..17739 + 1065 WP_012291478.1 AAA family ATPase -
BCD20_RS25635 18277..18699 - 423 WP_179364589.1 DNA distortion polypeptide 3 -
BCD20_RS25640 18767..19213 - 447 WP_085381553.1 hypothetical protein -
BCD20_RS25645 19253..20089 - 837 WP_001575533.1 replication initiation protein -
BCD20_RS25655 21349..21600 + 252 WP_000121743.1 plasmid stabilization protein Antitoxin
BCD20_RS25660 21590..21871 + 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BCD20_RS25665 22017..22340 + 324 WP_001181909.1 hypothetical protein -
BCD20_RS25670 22385..22630 + 246 WP_000356542.1 hypothetical protein -
BCD20_RS25675 22620..22835 + 216 WP_001180117.1 hypothetical protein -
BCD20_RS25680 22928..23257 + 330 WP_000866654.1 hypothetical protein -
BCD20_RS25685 23300..23479 + 180 WP_012301145.1 hypothetical protein -
BCD20_RS25695 23711..23983 + 273 WP_000160399.1 hypothetical protein -
BCD20_RS26275 24240..24314 + 75 Protein_30 DNA distortion polypeptide 3 -
BCD20_RS25700 24309..24854 + 546 WP_012301143.1 hypothetical protein -
BCD20_RS25705 24857..26023 + 1167 WP_122987139.1 relaxase/mobilization nuclease domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - mrkA / mrkB / mrkC / mrkD / mrkF 1..37362 37362


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T75488 WP_000220560.1 NZ_CP020836:21590-21871 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T75488 NZ_CP020836:21590-21871 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT75488 WP_000121743.1 NZ_CP020836:21349-21600 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT75488 NZ_CP020836:21349-21600 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References