Detailed information of TA system TA075482

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 3772501..3773180
• Replicon: chromosome
• Accession: NZ_CP020835
• Organism: Escherichia coli strain CFSAN051542

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: S1FHS4
• Locus tag: BCD20_RS19735
• Protein ID: WP_000854680.1
• Coordinates: 3772839..3773180 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: S1EWR7
• Locus tag: BCD20_RS19730
• Protein ID: WP_000070396.1
• Coordinates: 3772501..3772818 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCD20_RS19685	3767939..3768760	+	822	WP_085381467.1	DUF945 domain-containing protein	-
BCD20_RS19690	3768977..3769678	+	702	WP_023326104.1	WYL domain-containing protein	-
BCD20_RS19695	3769719..3769955	+	237	WP_057107659.1	hypothetical protein	-
BCD20_RS19700	3769955..3770398	+	444	WP_016239438.1	phage transcriptional regulator	-
BCD20_RS19705	3770422..3770889	+	468	WP_057107658.1	hypothetical protein	-
BCD20_RS19710	3770966..3771205	+	240	WP_000194654.1	DUF905 domain-containing protein	-
BCD20_RS19715	3771303..3771761	+	459	WP_000211838.1	antirestriction protein	-
BCD20_RS19720	3771777..3772252	+	476	Protein_3617	RadC family protein	-
BCD20_RS19725	3772261..3772482	+	222	WP_000691994.1	DUF987 domain-containing protein	-
BCD20_RS19730	3772501..3772818	+	318	WP_000070396.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
BCD20_RS19735	3772839..3773180	+	342	WP_000854680.1	type IV toxin-antitoxin system toxin YkfI	Toxin
BCD20_RS19740	3773720..3774934	+	1215	WP_033801639.1	tyrosine-type recombinase/integrase	-
BCD20_RS19745	3774970..3775248	-	279	WP_033801635.1	hypothetical protein	-
BCD20_RS19750	3775587..3777452	-	1866	WP_045148748.1	cell envelope integrity protein TolA	-
BCD20_RS19755	3777445..3777642	-	198	WP_033801633.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3761675..3810321	48646

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12892.95 Da
• Isoelectric Point: 9.6543

>T75482 WP_000854680.1 NZ_CP020835:3772839-3773180 [Escherichia coli]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T75482 NZ_CP020835:3772839-3773180 [Escherichia coli]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11982.62 Da
• Isoelectric Point: 7.0270

>AT75482 WP_000070396.1 NZ_CP020835:3772501-3772818 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT75482 NZ_CP020835:3772501-3772818 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: S1FHS4

Antitoxin

• AlphaFold DB: S1EWR7