Detailed information of TA system TA075470

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 2516755..2517281
• Replicon: chromosome
• Accession: NZ_CP020835
• Organism: Escherichia coli strain CFSAN051542

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: BCD20_RS13085
• Protein ID: WP_000323025.1
• Coordinates: 2516994..2517281 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: BCD20_RS13080
• Protein ID: WP_000534858.1
• Coordinates: 2516755..2516994 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCD20_RS13045	2512219..2512916	-	698	Protein_2396	IS1 family transposase	-
BCD20_RS26205	2513084..2513371	+	288	Protein_2397	hypothetical protein	-
BCD20_RS13065	2514140..2515453	+	1314	WP_000589005.1	ISNCY family transposase	-
BCD20_RS13070	2515890..2516222	-	333	WP_001301033.1	protein FlxA	-
BCD20_RS13075	2516425..2516730	-	306	WP_001326990.1	hypothetical protein	-
BCD20_RS13080	2516755..2516994	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
BCD20_RS13085	2516994..2517281	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
BCD20_RS13090	2517353..2517508	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
BCD20_RS13095	2517725..2517976	+	252	WP_000980994.1	hypothetical protein	-
BCD20_RS13100	2518043..2518321	+	279	WP_012304870.1	hypothetical protein	-
BCD20_RS13105	2518323..2519372	+	1050	WP_028985484.1	DUF968 domain-containing protein	-
BCD20_RS13110	2519386..2520138	+	753	WP_001047135.1	antitermination protein	-
BCD20_RS25920	2520416..2520505	-	90	WP_120795389.1	hypothetical protein	-
BCD20_RS13115	2520560..2520774	-	215	Protein_2409	cold shock-like protein CspF	-
BCD20_RS13120	2521075..2521290	+	216	WP_000066484.1	cold shock-like protein CspB	-
BCD20_RS26365	2521654..2521824	-	171	WP_211180497.1	putative zinc-binding protein YnfU	-
BCD20_RS13125	2522044..2522259	+	216	WP_000839590.1	phage lysis protein EssD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2498503..2539382	40879
	-	flank	IS/Tn	-	-	2512219..2512596	377

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T75470 WP_000323025.1 NZ_CP020835:2516994-2517281 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T75470 NZ_CP020835:2516994-2517281 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT75470 WP_000534858.1 NZ_CP020835:2516755-2516994 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT75470 NZ_CP020835:2516755-2516994 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8