Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 977255..977909 Replicon chromosome
Accession NZ_CP020835
Organism Escherichia coli strain CFSAN051542

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag BCD20_RS05145 Protein ID WP_000244781.1
Coordinates 977502..977909 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag BCD20_RS05140 Protein ID WP_000354046.1
Coordinates 977255..977521 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BCD20_RS05115 972543..973286 + 744 WP_000951964.1 SDR family oxidoreductase -
BCD20_RS05120 973343..974776 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
BCD20_RS05125 974821..975132 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
BCD20_RS05130 975296..975955 + 660 WP_000250274.1 hemolysin III family protein -
BCD20_RS05135 976032..977012 - 981 WP_000886083.1 tRNA-modifying protein YgfZ -
BCD20_RS05140 977255..977521 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
BCD20_RS05145 977502..977909 + 408 WP_000244781.1 protein YgfX Toxin
BCD20_RS05150 977949..978470 - 522 WP_044863754.1 flavodoxin FldB -
BCD20_RS05155 978582..979478 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
BCD20_RS05160 979503..980213 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
BCD20_RS05165 980219..981952 + 1734 WP_000813201.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T75467 WP_000244781.1 NZ_CP020835:977502-977909 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T75467 NZ_CP020835:977502-977909 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT75467 WP_000354046.1 NZ_CP020835:977255-977521 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT75467 NZ_CP020835:977255-977521 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References