Detailed information of TA system TA075460

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 3159520..3160185
• Replicon: chromosome
• Accession: NZ_CP020821
• Organism: Mycobacterium colombiense CECT 3035

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: B9D87_RS14435
• Protein ID: WP_007777097.1
• Coordinates: 3159520..3159993 (-)
• Length: 158 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: J4JTY5
• Locus tag: B9D87_RS14440
• Protein ID: WP_007777099.1
• Coordinates: 3159997..3160185 (-)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B9D87_RS14410	3154925..3156151	+	1227	WP_007777086.1	acetyl-CoA acetyltransferase	-
B9D87_RS14415	3156154..3157668	+	1515	WP_007777090.1	carotenoid oxygenase family protein	-
B9D87_RS14420	3157668..3158153	+	486	WP_007777091.1	VOC family protein	-
B9D87_RS14425	3158158..3158592	-	435	WP_007777094.1	hypothetical protein	-
B9D87_RS14430	3158661..3159431	-	771	WP_007777095.1	VOC family protein	-
B9D87_RS14435	3159520..3159993	-	474	WP_007777097.1	SRPBCC family protein	Toxin
B9D87_RS14440	3159997..3160185	-	189	WP_007777099.1	antitoxin	Antitoxin
B9D87_RS14445	3160252..3162633	-	2382	WP_007777101.1	cation-translocating P-type ATPase	-
B9D87_RS14450	3162630..3164213	-	1584	WP_007777105.1	serine hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-51)

Sequences

Toxin

Protein

• Length: 158 a.a.
• Molecular weight: 17004.53 Da
• Isoelectric Point: 7.4011

>T75460 WP_007777097.1 NZ_CP020821:c3159993-3159520 [Mycobacterium colombiense CECT 3035]
MAKLSGSIDVPLPPEVAWQHASDLSRYKDWLTIHRVWRSTLPDEIEKGTVVESIVEVKGMYSRIKWTVVRYKPPEGMTLN
GDGVGGVKVKLLAKIAPKDDGSVVSFDVHLGGPALFGPIGMIVAAALRGDINQSLENFVTVFTRADPSTNGDGGPHR

Nucleotide

• Length: 474 bp

>T75460 NZ_CP020821:c3159993-3159520 [Mycobacterium colombiense CECT 3035]
ATGGCGAAGCTTTCCGGATCCATCGATGTCCCGCTGCCGCCCGAGGTGGCCTGGCAGCACGCCTCCGACCTGTCCCGGTA
CAAGGACTGGCTGACCATTCACCGGGTGTGGCGCAGCACCCTGCCCGACGAGATCGAGAAGGGCACGGTCGTCGAATCGA
TCGTCGAGGTCAAGGGCATGTACAGCCGGATCAAATGGACGGTGGTGCGCTACAAGCCGCCCGAGGGCATGACGCTCAAC
GGCGACGGCGTCGGCGGGGTCAAGGTCAAGCTGCTGGCCAAGATCGCGCCCAAGGACGACGGCTCGGTCGTCAGCTTCGA
CGTGCACCTCGGCGGACCGGCGCTGTTCGGCCCCATCGGCATGATCGTCGCCGCCGCCCTGCGCGGGGACATCAACCAAT
CGCTGGAGAACTTCGTCACGGTGTTCACCCGCGCCGATCCGAGCACGAACGGAGACGGTGGGCCTCATCGCTGA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6758.42 Da
• Isoelectric Point: 4.4536

>AT75460 WP_007777099.1 NZ_CP020821:c3160185-3159997 [Mycobacterium colombiense CECT 3035]
MGFLDKAKDLLSQNADKVEMAIDKAGEFVDDKTQGKYTDTIHQVQEQAKKATGAADTGDQQN

Nucleotide

• Length: 189 bp

>AT75460 NZ_CP020821:c3160185-3159997 [Mycobacterium colombiense CECT 3035]
ATGGGATTCCTGGACAAGGCAAAAGACCTGCTGTCGCAGAACGCCGACAAGGTCGAAATGGCGATCGACAAGGCCGGCGA
ATTCGTCGACGACAAGACGCAGGGCAAATACACCGACACCATCCACCAGGTGCAGGAGCAGGCCAAGAAGGCCACCGGTG
CCGCTGACACCGGCGACCAGCAGAACTGA

Structures

Antitoxin

• AlphaFold DB: J4JTY5