Detailed information of TA system TA075459

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/ParD(antitoxin)
• Location: 1517572..1518570
• Replicon: chromosome
• Accession: NZ_CP020821
• Organism: Mycobacterium colombiense CECT 3035

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: J4SJ64
• Locus tag: B9D87_RS07135
• Protein ID: WP_007770932.1
• Coordinates: 1517572..1518150 (-)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: J5EMI8
• Locus tag: B9D87_RS07140
• Protein ID: WP_007770931.1
• Coordinates: 1518181..1518570 (-)
• Length: 130 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B9D87_RS07110	1513492..1514487	-	996	WP_007770941.1	sigma-70 family RNA polymerase sigma factor	-
B9D87_RS07115	1514657..1515007	+	351	WP_040630279.1	DoxX family protein	-
B9D87_RS07120	1515020..1515820	-	801	WP_040629807.1	nuclear transport factor 2 family protein	-
B9D87_RS07125	1515820..1516380	-	561	WP_007770935.1	pyrazinamidase PncA	-
B9D87_RS07130	1516482..1517549	+	1068	WP_052002472.1	DoxX family membrane protein	-
B9D87_RS07135	1517572..1518150	-	579	WP_007770932.1	zeta toxin family protein	Toxin
B9D87_RS07140	1518181..1518570	-	390	WP_007770931.1	hypothetical protein	Antitoxin
B9D87_RS07145	1518638..1519132	-	495	WP_007770930.1	gluconokinase	-
B9D87_RS07150	1519133..1521145	-	2013	WP_007770929.1	phosphotransferase	-
B9D87_RS07155	1521164..1522681	-	1518	WP_007770928.1	carboxylesterase/lipase family protein	-
B9D87_RS07160	1522776..1523435	+	660	WP_080598556.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (5-135)

Antitoxin

• ParD-like antitoxin of type II bacterial toxin-antitoxin system (6-106)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21155.22 Da
• Isoelectric Point: 10.1934

>T75459 WP_007770932.1 NZ_CP020821:c1518150-1517572 [Mycobacterium colombiense CECT 3035]
MDRLDLVVGPNGAGKSTFIAFTLAPLLPGSLVVNADEIARQRWPEDPASHSYDAARIAADTRAKLIDLGRSFVAETVFSH
PSKLDLIHTARHAGFTVVLHVLLVPEDLAVERVRHRVRAGGHDVPEVKIRERHRRLWTPVAEAIALSDLATVYDNSRLRG
PRIIARLSGGPTVGAPEWPAWTPENLRARWPA

Nucleotide

• Length: 579 bp

>T75459 NZ_CP020821:c1518150-1517572 [Mycobacterium colombiense CECT 3035]
ATGGACCGACTTGACCTGGTGGTCGGGCCGAACGGCGCCGGTAAATCCACCTTCATCGCCTTCACCCTGGCGCCGCTCTT
GCCGGGCAGCCTCGTGGTCAACGCCGACGAGATCGCCAGGCAACGCTGGCCGGAGGACCCGGCCTCGCACTCCTACGACG
CCGCGCGCATCGCCGCCGACACCCGCGCCAAGCTGATCGACCTGGGTCGGTCCTTCGTCGCCGAGACGGTGTTTTCCCAT
CCCTCCAAGCTGGACCTCATCCACACCGCGCGCCACGCGGGCTTCACCGTTGTGCTGCACGTGCTGCTCGTCCCGGAAGA
CCTTGCGGTGGAACGCGTCCGACACCGGGTGCGGGCCGGCGGCCACGATGTGCCGGAAGTCAAGATCCGCGAGCGCCACC
GCCGGTTGTGGACGCCGGTCGCCGAGGCCATCGCCTTGTCCGATCTCGCGACGGTGTATGACAACAGTCGGCTGCGCGGC
CCGCGGATCATCGCGCGGCTCAGCGGCGGCCCGACGGTCGGCGCGCCCGAGTGGCCGGCGTGGACGCCGGAAAACCTGCG
GGCTCGCTGGCCCGCGTAG

Antitoxin

Protein

• Length: 130 a.a.
• Molecular weight: 13501.95 Da
• Isoelectric Point: 5.1905

>AT75459 WP_007770931.1 NZ_CP020821:c1518570-1518181 [Mycobacterium colombiense CECT 3035]
MAEALDRVTRVASDLMDSAAAEGARQSRSAKQQLDHWARVGRAVSSQHTAPRRRVEAALAGQLATSELTVEEGVVFNAEI
SAAIEESLARTHYGATLAGQGVTTVALNDDGEIVEHRPDGAAVVLAGGR

Nucleotide

• Length: 390 bp

>AT75459 NZ_CP020821:c1518570-1518181 [Mycobacterium colombiense CECT 3035]
ATGGCCGAGGCTCTCGATCGTGTCACCAGGGTCGCATCCGACCTGATGGACAGCGCGGCGGCTGAGGGGGCCCGGCAGAG
CCGCTCGGCCAAGCAGCAACTCGACCACTGGGCGCGGGTCGGGCGAGCGGTGTCCAGCCAGCACACCGCCCCGCGGCGAC
GGGTGGAGGCCGCTCTGGCCGGCCAGCTCGCGACGAGCGAGCTCACCGTCGAAGAGGGCGTGGTGTTCAACGCCGAGATC
TCCGCGGCCATCGAAGAAAGCCTGGCGCGCACCCACTACGGGGCGACGCTGGCCGGGCAGGGGGTCACCACCGTCGCGCT
GAACGACGACGGCGAGATCGTCGAGCACCGCCCCGACGGCGCCGCGGTGGTGCTGGCGGGCGGGCGCTGA

Structures

Toxin

• AlphaFold DB: J4SJ64

Antitoxin

• AlphaFold DB: J5EMI8