Detailed information of TA system TA075458

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 1472021..1472502
• Replicon: chromosome
• Accession: NZ_CP020821
• Organism: Mycobacterium colombiense CECT 3035

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: J5EMS1
• Locus tag: B9D87_RS06885
• Protein ID: WP_007771023.1
• Coordinates: 1472170..1472502 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: B9D87_RS06880
• Protein ID: WP_167669436.1
• Coordinates: 1472021..1472173 (+)
• Length: 51 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B9D87_RS06865	1468029..1468967	+	939	WP_007771029.1	fumarylacetoacetate hydrolase family protein	-
B9D87_RS06870	1468964..1470547	+	1584	WP_040629814.1	bifunctional 3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylase	-
B9D87_RS06875	1470649..1471748	+	1100	Protein_1363	cytochrome P450	-
B9D87_RS06880	1472021..1472173	+	153	WP_167669436.1	antitoxin MazE family protein	Antitoxin
B9D87_RS06885	1472170..1472502	+	333	WP_007771023.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
B9D87_RS26480	1472930..1473049	+	120	Protein_1366	site-specific integrase	-
B9D87_RS06895	1473331..1473807	+	477	WP_007771020.1	MarR family transcriptional regulator	-
B9D87_RS06900	1473895..1474551	+	657	WP_007771019.1	SDR family oxidoreductase	-
B9D87_RS06905	1474572..1476131	+	1560	Protein_1369	aryl-sulfate sulfotransferase	-
B9D87_RS06915	1477228..1477458	-	231	WP_174320896.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1452978..1477458	24480

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-105)

Antitoxin

• Antitoxin MazE-like (1-48)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11833.69 Da
• Isoelectric Point: 8.7287

>T75458 WP_007771023.1 NZ_CP020821:1472170-1472502 [Mycobacterium colombiense CECT 3035]
VNRGEIWTVAGGVYATKPRPAVIVQDDLFAATSSVTVAPMSSTLLDAPLMRIRIAGGDGRLSGLDHDSDVMIDKLTTVKR
SNVHTRVGRLTAEQVVEIERAMMAFLGLAR

Nucleotide

• Length: 333 bp

>T75458 NZ_CP020821:1472170-1472502 [Mycobacterium colombiense CECT 3035]
GTGAACAGAGGGGAGATCTGGACCGTAGCGGGGGGTGTCTACGCGACCAAACCGCGTCCCGCGGTCATTGTTCAGGATGA
TCTCTTTGCTGCCACGAGTTCAGTGACGGTTGCTCCGATGTCCAGCACGCTGTTGGACGCGCCGTTGATGCGCATCCGGA
TAGCCGGCGGAGACGGCCGGTTATCCGGACTTGATCACGACAGTGACGTGATGATCGACAAGCTCACAACAGTCAAAAGA
TCGAACGTCCACACACGAGTCGGTCGGCTGACAGCGGAGCAAGTCGTCGAGATCGAGCGAGCGATGATGGCATTCCTCGG
CCTTGCGCGATAG

Antitoxin

Protein

• Length: 51 a.a.
• Molecular weight: 5717.19 Da
• Isoelectric Point: 3.9013

>AT75458 WP_167669436.1 NZ_CP020821:1472021-1472173 [Mycobacterium colombiense CECT 3035]
MRPLQVWVPDVRTESFAAEAHRQASLVARADESTDDQDFIEAISTPWDEE

Nucleotide

• Length: 153 bp

>AT75458 NZ_CP020821:1472021-1472173 [Mycobacterium colombiense CECT 3035]
CTGCGGCCCCTGCAGGTCTGGGTGCCTGACGTGCGCACTGAGAGTTTTGCCGCCGAGGCGCATCGGCAGGCTTCGTTGGT
CGCACGAGCGGACGAAAGCACCGATGACCAGGACTTCATTGAGGCAATCTCGACGCCATGGGACGAGGAGTGA

Structures

Toxin

• AlphaFold DB: J5EMS1