Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3066739..3067356 Replicon chromosome
Accession NZ_CP020820
Organism Pantoea vagans strain FBS135

Toxin (Protein)


Gene name Hha Uniprot ID E0LTU7
Locus tag B9D02_RS14655 Protein ID WP_003850458.1
Coordinates 3067141..3067356 (+) Length 72 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID E0LTU8
Locus tag B9D02_RS14650 Protein ID WP_003850455.1
Coordinates 3066739..3067116 (+) Length 126 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B9D02_RS14620 3063049..3063309 + 261 WP_008924911.1 type B 50S ribosomal protein L31 -
B9D02_RS14625 3063313..3063453 + 141 WP_003850445.1 type B 50S ribosomal protein L36 -
B9D02_RS14630 3063499..3064377 - 879 WP_039659090.1 metal ABC transporter substrate-binding protein -
B9D02_RS14635 3064393..3065232 - 840 WP_003850449.1 metal ABC transporter permease -
B9D02_RS14640 3065229..3065897 - 669 WP_008924910.1 ABC transporter ATP-binding protein -
B9D02_RS14645 3066238..3066591 + 354 WP_003850454.1 hypothetical protein -
B9D02_RS14650 3066739..3067116 + 378 WP_003850455.1 Hha toxicity modulator TomB Antitoxin
B9D02_RS14655 3067141..3067356 + 216 WP_003850458.1 hemolysin expression modulator Hha Toxin
B9D02_RS14665 3067776..3068093 + 318 WP_039659087.1 MGMT family protein -
B9D02_RS14670 3068124..3068681 - 558 WP_008924908.1 YbaY family lipoprotein -
B9D02_RS14675 3068872..3069735 + 864 WP_003850463.1 acyl-CoA thioesterase II -
B9D02_RS14680 3069784..3071070 - 1287 WP_003850465.1 ammonium transporter AmtB -
B9D02_RS14685 3071105..3071443 - 339 WP_003850469.1 P-II family nitrogen regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(12-68)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 72 a.a.        Molecular weight: 8510.90 Da        Isoelectric Point: 9.4825

>T75452 WP_003850458.1 NZ_CP020820:3067141-3067356 [Pantoea vagans]
MNKTLTKTDYLMRLRRCRSLDTLERVIEKNKYELPEDELAVFYSAADHRLAELTMNKLYDKVPGSVWKFVR

Download         Length: 216 bp

>T75452 NZ_CP020820:3067141-3067356 [Pantoea vagans]
ATGAACAAAACCCTGACTAAAACTGATTATTTGATGCGACTACGGCGTTGCCGTTCACTGGATACCCTGGAAAGGGTCAT
TGAAAAAAATAAGTATGAATTACCTGAAGATGAGTTAGCGGTATTTTACTCGGCAGCTGACCATCGTCTGGCAGAACTGA
CGATGAACAAGCTTTACGATAAAGTCCCGGGTTCAGTCTGGAAATTTGTGCGCTAG

Antitoxin


Download         Length: 126 a.a.        Molecular weight: 14636.25 Da        Isoelectric Point: 4.3976

>AT75452 WP_003850455.1 NZ_CP020820:3066739-3067116 [Pantoea vagans]
MDEYSPKRHDIAQLKYLCESLFDDSMATLTDSHHGWVNDPTSESNLQLNDLIEHIASFTMNYKIKHVEDEALISQIDEYL
DDTFMLFSNYGVNSPDLQRWQRSAKRLFNLFTEECAFLQQPSHSL

Download         Length: 378 bp

>AT75452 NZ_CP020820:3066739-3067116 [Pantoea vagans]
ATGGACGAATACTCACCGAAACGGCATGATATTGCCCAGCTTAAATACCTATGTGAAAGCCTGTTTGACGACAGCATGGC
AACATTAACCGACAGCCATCATGGCTGGGTAAATGATCCCACTTCTGAAAGCAATCTGCAGCTTAACGATTTGATTGAGC
ACATCGCCTCTTTCACCATGAATTACAAAATTAAGCACGTTGAAGATGAAGCGCTGATTTCGCAAATCGATGAATATCTT
GATGATACCTTTATGTTATTCAGCAACTATGGTGTCAATAGTCCGGATCTTCAACGGTGGCAACGTTCAGCTAAACGCTT
ATTTAATCTGTTCACTGAAGAGTGCGCTTTTCTCCAGCAACCGAGCCATTCATTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1I5AGC3

Antitoxin

Source ID Structure
AlphaFold DB A0A1I5AG55

References