Detailed information of TA system TA075444

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpA-brnA/BrnT_toxin-BrnA
• Location: 3192216..3192747
• Replicon: chromosome
• Accession: NZ_CP020818
• Organism: Bordetella bronchiseptica strain F709

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: B9G73_RS15065
• Protein ID: WP_003816744.1
• Coordinates: 3192216..3192482 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: K0MF02
• Locus tag: B9G73_RS15070
• Protein ID: WP_003816743.1
• Coordinates: 3192469..3192747 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B9G73_RS15040	3187450..3188160	-	711	WP_033448180.1	aquaporin Z	-
B9G73_RS15045	3188268..3188900	-	633	WP_003812619.1	PAS domain-containing protein	-
B9G73_RS15050	3189297..3190175	+	879	WP_003816745.1	tripartite tricarboxylate transporter substrate binding protein	-
B9G73_RS15055	3190237..3191779	+	1543	Protein_2963	M81 family metallopeptidase	-
B9G73_RS15060	3191907..3192098	+	192	Protein_2964	IS481 family transposase	-
B9G73_RS15065	3192216..3192482	+	267	WP_003816744.1	BrnT family toxin	Toxin
B9G73_RS15070	3192469..3192747	+	279	WP_003816743.1	BrnA antitoxin family protein	Antitoxin
B9G73_RS15080	3193046..3193702	-	657	WP_003816742.1	phosphoribosylanthranilate isomerase	-
B9G73_RS15085	3193717..3195384	-	1668	WP_015041648.1	TRAP transporter large permease subunit	-
B9G73_RS15090	3195387..3196016	-	630	WP_010930386.1	TRAP transporter small permease subunit	-
B9G73_RS15095	3196228..3197322	-	1095	WP_003812637.1	TRAP transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (12-86)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10032.44 Da
• Isoelectric Point: 9.4798

>T75444 WP_003816744.1 NZ_CP020818:3192216-3192482 [Bordetella bronchiseptica]
MDITYDPAKNDKNIADRGLSFELVRGFEWASALVVEDARQAYAETRYQALGKIDGRLHMVVFTVRGESLRIISLRKANAR
EVARYEKA

Nucleotide

• Length: 267 bp

>T75444 NZ_CP020818:3192216-3192482 [Bordetella bronchiseptica]
ATGGATATCACCTACGACCCCGCCAAGAACGACAAGAACATTGCCGATCGCGGCCTGTCGTTCGAGTTGGTGCGTGGCTT
CGAGTGGGCCAGCGCGCTGGTGGTTGAGGATGCCCGCCAGGCCTACGCCGAAACGCGCTATCAGGCGCTGGGCAAAATCG
ATGGCCGGCTCCATATGGTGGTATTCACGGTGCGCGGCGAGTCGCTGCGCATCATCAGCCTGCGTAAGGCAAATGCTCGT
GAGGTAGCTCGTTATGAAAAAGCCTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10412.89 Da
• Isoelectric Point: 10.5548

>AT75444 WP_003816743.1 NZ_CP020818:3192469-3192747 [Bordetella bronchiseptica]
MKKPDPEKIDRDNPEWTDAEFRRAKPAAEVLPESVHAMLGIRRRGPQKTPTKQVVTIRLSPDVVEAFKASGTGWQTRVDA
ALRDWLKTHQPG

Nucleotide

• Length: 279 bp

>AT75444 NZ_CP020818:3192469-3192747 [Bordetella bronchiseptica]
ATGAAAAAGCCTGACCCCGAAAAGATTGACCGCGACAACCCCGAATGGACGGACGCGGAATTTCGGCGGGCCAAGCCGGC
CGCCGAAGTACTGCCCGAGTCGGTGCACGCGATGCTCGGCATTCGTCGCCGTGGGCCTCAAAAGACACCCACCAAACAGG
TCGTCACCATCCGGCTATCGCCTGACGTGGTAGAAGCCTTCAAGGCCTCCGGCACCGGTTGGCAGACTCGCGTGGATGCA
GCCCTGCGGGATTGGCTGAAGACGCACCAGCCTGGCTAG

Structures

Antitoxin

• AlphaFold DB: K0MF02