Detailed information of TA system TA075439

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-RHH
• Location: 4552674..4553290
• Replicon: chromosome
• Accession: NZ_CP020817
• Organism: Enterobacter sp. Crenshaw

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: B7P19_RS22150
• Protein ID: WP_080298642.1
• Coordinates: 4552674..4553045 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A808IJD0
• Locus tag: B7P19_RS22155
• Protein ID: WP_023309753.1
• Coordinates: 4553048..4553290 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7P19_RS22135	4550174..4551076	+	903	WP_008501831.1	formate dehydrogenase subunit beta	-
B7P19_RS22140	4551073..4551708	+	636	WP_010436852.1	formate dehydrogenase cytochrome b556 subunit	-
B7P19_RS22145	4551705..4552634	+	930	WP_029741588.1	formate dehydrogenase accessory protein FdhE	-
B7P19_RS22150	4552674..4553045	-	372	WP_080298642.1	type II toxin-antitoxin system VapC family toxin	Toxin
B7P19_RS22155	4553048..4553290	-	243	WP_023309753.1	ribbon-helix-helix protein, CopG family	Antitoxin
B7P19_RS22160	4553490..4554410	+	921	WP_048981029.1	alpha/beta hydrolase	-
B7P19_RS22165	4554419..4555360	-	942	WP_039264191.1	fatty acid biosynthesis protein FabY	-
B7P19_RS22170	4555405..4555842	-	438	WP_008501836.1	D-tyrosyl-tRNA(Tyr) deacylase	-
B7P19_RS22175	4555839..4556720	-	882	WP_033146965.1	virulence factor BrkB family protein	-
B7P19_RS22180	4556714..4557313	-	600	WP_039264189.1	glucose-1-phosphatase	-
B7P19_RS22185	4557402..4557872	-	471	WP_033146967.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-109)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13688.85 Da
• Isoelectric Point: 6.9789

>T75439 WP_080298642.1 NZ_CP020817:c4553045-4552674 [Enterobacter sp. Crenshaw]
MEHMAVFDTNILIDLFNNRVEAADAIDRTASHRAISLITWMEVMVGARKHGHEAKTATVMGVFEIIDITRDIAERSVILR
EKHGMKLPDAIILATAQSRNCPLISRNTKDFSGITGVVSPYQL

Nucleotide

• Length: 372 bp

>T75439 NZ_CP020817:c4553045-4552674 [Enterobacter sp. Crenshaw]
ATGGAGCACATGGCAGTGTTCGATACCAATATACTCATCGATCTTTTTAATAATCGCGTTGAGGCTGCGGATGCAATTGA
TCGCACAGCTTCTCATCGAGCCATTAGTCTGATCACATGGATGGAAGTCATGGTGGGGGCCCGCAAACATGGTCACGAAG
CGAAAACTGCGACAGTGATGGGCGTATTTGAGATTATTGATATCACCCGAGATATCGCTGAAAGAAGTGTAATTCTGCGC
GAGAAGCATGGAATGAAACTGCCTGATGCTATCATTCTGGCCACAGCTCAGAGCAGAAATTGTCCGTTGATTTCTCGAAA
CACGAAAGACTTTTCGGGCATTACTGGAGTCGTTTCACCTTACCAACTATAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9411.53 Da
• Isoelectric Point: 4.2803

>AT75439 WP_023309753.1 NZ_CP020817:c4553290-4553048 [Enterobacter sp. Crenshaw]
ISLSADLDMGRILIDLSDDVIQRLDNLKQLRNRPRAELLREAIEQYLDQQSSSVIRDALGLWGNQQEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT75439 NZ_CP020817:c4553290-4553048 [Enterobacter sp. Crenshaw]
ATAAGTCTTTCCGCGGATCTTGATATGGGCAGAATTCTGATCGATTTGTCTGATGACGTAATTCAACGACTGGATAATCT
GAAGCAGCTGCGTAACCGTCCTCGTGCCGAATTGCTTCGGGAAGCCATTGAGCAATATCTCGACCAGCAGAGTTCATCGG
TTATCCGCGATGCGCTTGGCTTGTGGGGAAACCAGCAGGAAGATGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A808IJD0