Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 577651..578227 Replicon chromosome
Accession NZ_CP020817
Organism Enterobacter sp. Crenshaw

Toxin (Protein)


Gene name shpA Uniprot ID A0A808IJQ6
Locus tag B7P19_RS02735 Protein ID WP_048980720.1
Coordinates 577651..577938 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A808I9W7
Locus tag B7P19_RS02740 Protein ID WP_048980603.1
Coordinates 577925..578227 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B7P19_RS02710 572752..573447 + 696 WP_048980606.1 winged helix-turn-helix domain-containing protein -
B7P19_RS02715 573597..574067 + 471 WP_033144545.1 MarR family transcriptional regulator -
B7P19_RS02720 574064..575131 + 1068 WP_101744430.1 HlyD family secretion protein -
B7P19_RS02725 575136..576212 + 1077 WP_023310256.1 DUF2955 domain-containing protein -
B7P19_RS02730 576209..577480 - 1272 WP_048980604.1 DUF445 domain-containing protein -
B7P19_RS02735 577651..577938 + 288 WP_048980720.1 BrnT family toxin Toxin
B7P19_RS02740 577925..578227 + 303 WP_048980603.1 BrnA antitoxin family protein Antitoxin
B7P19_RS02745 578258..578896 - 639 WP_048980602.1 LysE family translocator -
B7P19_RS02750 578944..579687 - 744 WP_048980600.1 AraC family transcriptional regulator -
B7P19_RS02755 579839..581209 + 1371 WP_048980598.1 NAD-dependent succinate-semialdehyde dehydrogenase -
B7P19_RS02760 581255..581575 - 321 WP_039262264.1 hypothetical protein -
B7P19_RS02765 581575..582120 - 546 WP_023310269.1 porin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(32-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11201.66 Da        Isoelectric Point: 6.7609

>T75425 WP_048980720.1 NZ_CP020817:577651-577938 [Enterobacter sp. Crenshaw]
MPTEFEWDTNKAKSNLIKHGIRFEEAVLVFDDPYHLSLQDRHENGEFRWQTIGLVHGLIVIMVAHTVRFESGDEVIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T75425 NZ_CP020817:577651-577938 [Enterobacter sp. Crenshaw]
ATGCCAACGGAGTTTGAATGGGATACCAACAAAGCGAAAAGCAATCTCATTAAGCATGGGATTCGCTTTGAAGAAGCTGT
TCTGGTGTTTGACGATCCTTACCATTTATCTTTGCAGGATCGTCATGAAAATGGAGAGTTCCGTTGGCAGACCATTGGCC
TTGTACATGGTCTAATTGTCATTATGGTCGCACACACGGTACGTTTTGAAAGTGGTGACGAAGTCATTCGAATCATTAGC
GCACGAAAGGCGGACCGTAAAGAGAGGAACCGTTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11451.16 Da        Isoelectric Point: 10.1522

>AT75425 WP_048980603.1 NZ_CP020817:577925-578227 [Enterobacter sp. Crenshaw]
MSMVKYKRGSAPEMSARREAELKALAEKSEAEIDYSDIPAMGDEQWSGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT75425 NZ_CP020817:577925-578227 [Enterobacter sp. Crenshaw]
ATGAGCATGGTTAAATATAAACGCGGTAGTGCGCCTGAGATGAGTGCTCGCCGTGAGGCTGAGCTCAAAGCACTAGCCGA
AAAATCGGAAGCAGAGATTGATTACAGCGATATCCCTGCCATGGGAGATGAACAGTGGTCAGGGGCGGTGCGAGGGAAGT
TCTATCGGCCACTCAAAACTCAGGCGTCTGTACGTATCGACGCTGACGTGATGGAGTGGCTTAAACGGCCTGGAAAAGGT
TACCAGACGAGGCTCAATGCCATTTTGCGTGAAGCTATGCTGCGCGATCAAAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A808IJQ6

Antitoxin

Source ID Structure
AlphaFold DB A0A808I9W7

References