Detailed information of TA system TA075423

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 233398..234030
• Replicon: chromosome
• Accession: NZ_CP020814
• Organism: Alkalihalobacillus krulwichiae strain AM31D

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: W4Q322
• Locus tag: BkAM31D_RS01235
• Protein ID: WP_034631315.1
• Coordinates: 233680..234030 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: BkAM31D_RS01230
• Protein ID: WP_174521931.1
• Coordinates: 233398..233676 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BkAM31D_RS01205	228465..229340	+	876	WP_157076914.1	metal ABC transporter substrate-binding protein	-
BkAM31D_RS01210	229410..230153	-	744	WP_066159437.1	rhomboid family intramembrane serine protease	-
BkAM31D_RS01215	230280..230642	+	363	WP_066159440.1	holo-ACP synthase	-
BkAM31D_RS01220	230866..231876	+	1011	WP_066159443.1	outer membrane lipoprotein carrier protein LolA	-
BkAM31D_RS01225	232085..233218	+	1134	WP_066159446.1	alanine racemase	-
BkAM31D_RS01230	233398..233676	+	279	WP_174521931.1	CopG family ribbon-helix-helix protein	Antitoxin
BkAM31D_RS01235	233680..234030	+	351	WP_034631315.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BkAM31D_RS01240	234289..235107	+	819	WP_174521932.1	STAS domain-containing protein	-
BkAM31D_RS01245	235123..235479	+	357	WP_066159454.1	STAS domain-containing protein	-
BkAM31D_RS01250	235482..235895	+	414	WP_066159457.1	anti-sigma regulatory factor	-
BkAM31D_RS01255	235908..236918	+	1011	WP_066159459.1	PP2C family protein-serine/threonine phosphatase	-
BkAM31D_RS01260	236971..237294	+	324	WP_066159461.1	STAS domain-containing protein	-
BkAM31D_RS01265	237291..237776	+	486	WP_066159463.1	anti-sigma B factor RsbW	-
BkAM31D_RS01270	237739..238523	+	785	Protein_227	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-109)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12980.00 Da
• Isoelectric Point: 6.4662

>T75423 WP_034631315.1 NZ_CP020814:233680-234030 [Alkalihalobacillus krulwichiae]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEINAKRYGFDRDSVILLEQV
RTIDKQRLTDKITHLDDDMMNRVNDALFISLGLIDF

Nucleotide

• Length: 351 bp

>T75423 NZ_CP020814:233680-234030 [Alkalihalobacillus krulwichiae]
TTGATTGTAAAAAGAGGCGACGTTTACTTTGCTGATCTCTCACCTGTTGTTGGTTCTGAACAAGGTGGAGTTCGTCCTGT
GCTTGTTATACAAAATGATATTGGAAACCGTTTTAGTCCTACGGTTATTGTGGCAGCAATTACAGCTCAAATTCAAAAAG
CTAAACTGCCAACGCATGTTGAGATTAATGCAAAACGTTATGGCTTTGATCGTGATTCCGTCATTTTGTTGGAACAAGTA
CGAACAATTGATAAGCAAAGGCTAACCGATAAAATAACCCACTTAGACGATGATATGATGAATCGTGTGAATGATGCTCT
GTTCATAAGTTTAGGACTTATTGATTTTTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10667.19 Da
• Isoelectric Point: 7.0162

>AT75423 WP_174521931.1 NZ_CP020814:233398-233676 [Alkalihalobacillus krulwichiae]
VSEQSTKRIVINLPQHLLNEVDGVIKKENVNRSEFISQATKMYLRERKKRQIRETMQQGYMEMAKINLNIASESFLAEEE
AENTLDRLVSGV

Nucleotide

• Length: 279 bp

>AT75423 NZ_CP020814:233398-233676 [Alkalihalobacillus krulwichiae]
GTGTCAGAGCAAAGCACAAAGCGCATTGTAATTAATTTGCCACAACATTTGTTGAACGAGGTGGACGGTGTGATTAAAAA
AGAGAACGTCAATCGTAGTGAATTCATTTCACAAGCAACAAAGATGTATCTTCGTGAACGTAAAAAACGTCAAATTCGGG
AGACTATGCAGCAAGGCTATATGGAAATGGCGAAAATTAACCTAAACATTGCATCAGAATCTTTTCTTGCCGAGGAGGAA
GCAGAGAATACCCTCGACCGCTTAGTGAGTGGGGTGTAG

Structures

Toxin

• AlphaFold DB: W4Q322