Detailed information of TA system TA075420

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 6084456..6085085
• Replicon: chromosome
• Accession: NZ_CP020809
• Organism: Mycobacterium dioxanotrophicus strain PH-06

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: BTO20_RS29570
• Protein ID: WP_087079455.1
• Coordinates: 6084456..6084902 (-)
• Length: 149 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: -
• Locus tag: BTO20_RS29575
• Protein ID: WP_087079456.1
• Coordinates: 6084906..6085085 (-)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BTO20_RS29545	6080894..6081136	+	243	WP_083164979.1	hypothetical protein	-
BTO20_RS29550	6081159..6082898	+	1740	WP_087079451.1	fumarate reductase (quinol) flavoprotein subunit	-
BTO20_RS29555	6082905..6083657	+	753	WP_087079452.1	succinate dehydrogenase/fumarate reductase iron-sulfur subunit	-
BTO20_RS29560	6083654..6084034	+	381	WP_087079453.1	fumarate reductase subunit C	-
BTO20_RS29565	6084031..6084387	+	357	WP_087079454.1	fumarate reductase subunit FrdD	-
BTO20_RS29570	6084456..6084902	-	447	WP_087079455.1	SRPBCC family protein	Toxin
BTO20_RS29575	6084906..6085085	-	180	WP_087079456.1	antitoxin	Antitoxin
BTO20_RS29580	6085157..6087532	-	2376	WP_087079457.1	cation-translocating P-type ATPase	-
BTO20_RS29585	6087593..6089197	-	1605	WP_087079458.1	serine hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-145)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-52)

Sequences

Toxin

Protein

• Length: 149 a.a.
• Molecular weight: 15999.62 Da
• Isoelectric Point: 9.8774

>T75420 WP_087079455.1 NZ_CP020809:c6084902-6084456 [Mycobacterium dioxanotrophicus]
MAKLSVSIDVPLPPEKAWEYASDLSRYDEWLSIHRAWRSKLPETLEKGTVIDSIVEVKGMLNRVKWTLVNYKPPQSLTLN
GDGRGGVKVKLIGKIKPATVDGGTGASVTFDVHLGGPALFGPIGMVVAAALKGDIQESLNKFKALYAP

Nucleotide

• Length: 447 bp

>T75420 NZ_CP020809:c6084902-6084456 [Mycobacterium dioxanotrophicus]
ATGGCGAAACTTTCTGTCTCCATTGATGTTCCACTCCCGCCCGAGAAGGCATGGGAGTACGCCTCCGACCTGTCCCGGTA
CGACGAGTGGTTGAGCATTCACCGGGCGTGGCGGTCGAAGCTGCCGGAAACTCTGGAAAAGGGCACGGTCATCGACTCGA
TCGTCGAGGTCAAGGGCATGTTGAACCGCGTCAAGTGGACGCTCGTCAATTACAAGCCACCGCAGTCGCTGACCCTTAAC
GGTGACGGCCGGGGCGGGGTCAAGGTCAAGCTGATCGGAAAGATCAAGCCGGCGACGGTCGACGGCGGCACCGGCGCCAG
CGTCACGTTCGACGTGCACCTCGGTGGTCCGGCGTTGTTCGGGCCCATCGGCATGGTGGTGGCGGCGGCCCTCAAGGGGG
ACATTCAGGAGTCCCTGAACAAGTTCAAGGCCCTCTACGCCCCGTAA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6364.07 Da
• Isoelectric Point: 4.5086

>AT75420 WP_087079456.1 NZ_CP020809:c6085085-6084906 [Mycobacterium dioxanotrophicus]
MSFLDKAKDLLAQNADKVESAIDKAGDMVDEKTEGKFSGVVDKAQDAAKNAIHKDEPTQ

Nucleotide

• Length: 180 bp

>AT75420 NZ_CP020809:c6085085-6084906 [Mycobacterium dioxanotrophicus]
ATGAGTTTCCTGGACAAGGCCAAGGATCTGCTGGCGCAGAACGCCGACAAGGTGGAATCCGCGATCGACAAGGCAGGCGA
CATGGTCGACGAGAAGACCGAGGGCAAGTTTTCTGGCGTTGTGGACAAGGCACAGGATGCCGCCAAGAACGCCATCCACA
AGGACGAACCGACGCAGTAA