Detailed information of TA system TA075415

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1746182..1747099
• Replicon: chromosome
• Accession: NZ_CP020805
• Organism: Bacillus velezensis strain 9D-6

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: B7941_RS09030
• Protein ID: WP_007407256.1
• Coordinates: 1746353..1747099 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: B7941_RS09025
• Protein ID: WP_003154807.1
• Coordinates: 1746182..1746352 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7941_RS08985	1741298..1742098	+	801	WP_060674874.1	ATP-binding protein	-
B7941_RS20005	1742098..1742265	+	168	WP_003154863.1	hypothetical protein	-
B7941_RS08990	1742363..1742704	+	342	WP_007407281.1	hypothetical protein	-
B7941_RS08995	1742694..1742897	+	204	WP_064107736.1	XtrA/YqaO family protein	-
B7941_RS09000	1743004..1743516	+	513	WP_059367159.1	sigma-70 family RNA polymerase sigma factor	-
B7941_RS09005	1743693..1744442	+	750	WP_085341919.1	phage portal protein	-
B7941_RS09010	1744494..1744757	+	264	WP_003154815.1	hemolysin XhlA family protein	-
B7941_RS09015	1744771..1745034	+	264	WP_003154813.1	phage holin	-
B7941_RS09020	1745048..1745926	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
B7941_RS09025	1746182..1746352	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
B7941_RS09030	1746353..1747099	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
B7941_RS09035	1747204..1748202	-	999	WP_020955696.1	inorganic phosphate transporter	-
B7941_RS09040	1748215..1748832	-	618	WP_003154804.1	DUF47 domain-containing protein	-
B7941_RS09045	1749118..1750434	-	1317	WP_085341920.1	amino acid permease	-
B7941_RS09050	1750757..1751707	+	951	WP_012117369.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1720665..1755364	34699

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T75415 WP_007407256.1 NZ_CP020805:c1747099-1746353 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T75415 NZ_CP020805:c1747099-1746353 [Bacillus velezensis]
ATGCTTTTGTTTTTTCAGATCATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGATTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCGGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTACTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT75415 WP_003154807.1 NZ_CP020805:c1746352-1746182 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT75415 NZ_CP020805:c1746352-1746182 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14