Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 989815..990452 Replicon chromosome
Accession NZ_CP020805
Organism Bacillus velezensis strain 9D-6

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag B7941_RS05040 Protein ID WP_003156187.1
Coordinates 990102..990452 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag B7941_RS05035 Protein ID WP_003156188.1
Coordinates 989815..990096 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B7941_RS05015 986180..986779 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
B7941_RS05020 986872..987237 + 366 WP_007410229.1 holo-ACP synthase -
B7941_RS05025 987402..988409 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
B7941_RS05030 988526..989695 + 1170 WP_025284272.1 alanine racemase -
B7941_RS05035 989815..990096 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
B7941_RS05040 990102..990452 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
B7941_RS05045 990570..991391 + 822 WP_007410232.1 STAS domain-containing protein -
B7941_RS05050 991396..991761 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
B7941_RS05055 991764..992165 + 402 WP_003156178.1 anti-sigma regulatory factor -
B7941_RS05060 992177..993184 + 1008 WP_007410233.1 PP2C family protein-serine/threonine phosphatase -
B7941_RS05065 993248..993577 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
B7941_RS05070 993574..994056 + 483 WP_007609591.1 anti-sigma B factor RsbW -
B7941_RS05075 994022..994810 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
B7941_RS05080 994810..995412 + 603 WP_007410234.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T75414 WP_003156187.1 NZ_CP020805:990102-990452 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T75414 NZ_CP020805:990102-990452 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT75414 WP_003156188.1 NZ_CP020805:989815-990096 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT75414 NZ_CP020805:989815-990096 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References