Detailed information of TA system TA075412

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 471194..471718
• Replicon: chromosome
• Accession: NZ_CP020773
• Organism: Staphylococcus lutrae strain ATCC 700373

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A380G4V0
• Locus tag: B5P37_RS02360
• Protein ID: WP_019169266.1
• Coordinates: 471194..471547 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: B5P37_RS02365
• Protein ID: WP_085236713.1
• Coordinates: 471548..471718 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B5P37_RS02335	467129..468448	+	1320	WP_085238400.1	ISL3 family transposase	-
B5P37_RS02340	468499..469269	-	771	WP_085236706.1	RNA polymerase sigma factor SigB	-
B5P37_RS02345	469244..469723	-	480	WP_085238401.1	anti-sigma B factor RsbW	-
B5P37_RS02350	469728..470054	-	327	WP_085236708.1	anti-sigma factor antagonist	-
B5P37_RS02355	470133..471137	-	1005	WP_085236711.1	PP2C family protein-serine/threonine phosphatase	-
B5P37_RS02360	471194..471547	-	354	WP_019169266.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
B5P37_RS02365	471548..471718	-	171	WP_085236713.1	antitoxin MazE	Antitoxin
B5P37_RS02370	471803..472951	-	1149	WP_085236715.1	alanine racemase	-
B5P37_RS02375	472985..473335	-	351	WP_085236717.1	holo-ACP synthase	-
B5P37_RS02380	473332..473841	-	510	WP_103322025.1	PH domain-containing protein	-
B5P37_RS02385	473847..475349	-	1503	WP_085236722.1	PH domain-containing protein	-
B5P37_RS02390	475342..475815	-	474	WP_085236725.1	PH domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-108)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13116.33 Da
• Isoelectric Point: 10.4439

>T75412 WP_019169266.1 NZ_CP020773:c471547-471194 [Staphylococcus lutrae]
MKRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKNKYKLDKDSVILLEQIRT
VDKKRLKEKLTFLSDKKMKEVNNAIGISLGLHMNHHK

Nucleotide

• Length: 354 bp

>T75412 NZ_CP020773:c471547-471194 [Staphylococcus lutrae]
ATGAAGCGCGGAGATGTTTATTTAGCAGATTTATCACCAGTTCAAGGGTCCGAACAAGGGGGAGTAAGACCCGTAGTCAT
TATTCAAAATGATACTGGAAATAAATATAGTCCCACAGTGATTGTTGCCGCGATTACCGGACGGATTAATAAGGCAAAAA
TACCAACGCATGTCGAAATTGAAAAAAATAAATATAAGCTCGATAAAGATTCTGTTATATTACTTGAACAAATTCGGACA
GTCGATAAAAAAAGGCTGAAAGAAAAGTTGACTTTTTTGTCTGATAAGAAGATGAAAGAAGTAAACAATGCAATTGGTAT
TAGTTTAGGACTGCACATGAATCATCATAAATAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6424.13 Da
• Isoelectric Point: 3.9930

>AT75412 WP_085236713.1 NZ_CP020773:c471718-471548 [Staphylococcus lutrae]
MLAFNQIRYKNIEQSLKEGYVQMADVNLSLATEAYSVECEACDCNESHLISDYKED

Nucleotide

• Length: 171 bp

>AT75412 NZ_CP020773:c471718-471548 [Staphylococcus lutrae]
ATGCTAGCATTCAATCAAATTAGGTATAAAAATATCGAACAATCATTAAAAGAAGGTTATGTTCAGATGGCAGATGTAAA
TCTCTCCCTAGCGACTGAAGCATACTCAGTTGAATGTGAAGCTTGCGATTGTAATGAATCACACTTGATATCTGACTATA
AGGAAGACTAG

Structures

Toxin

• AlphaFold DB: A0A380G4V0