Detailed information of TA system TA075411

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF(toxin)
• Location: 3880131..3880768
• Replicon: chromosome
• Accession: NZ_CP020772
• Organism: Halobacillus mangrovi strain KTB 131

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: HM131_RS19310
• Protein ID: WP_085032136.1
• Coordinates: 3880131..3880481 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A0B0D213
• Locus tag: HM131_RS19315
• Protein ID: WP_035549809.1
• Coordinates: 3880487..3880768 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HM131_RS19275	3875613..3876392	-	780	WP_085031289.1	RNA polymerase sigma factor SigB	-
HM131_RS19280	3876364..3876840	-	477	WP_085031290.1	anti-sigma B factor RsbW	-
HM131_RS19285	3876843..3877172	-	330	WP_035542130.1	STAS domain-containing protein	-
HM131_RS19290	3877238..3878248	-	1011	WP_085031291.1	PP2C family protein-serine/threonine phosphatase	-
HM131_RS19295	3878261..3878662	-	402	WP_085031292.1	anti-sigma regulatory factor	-
HM131_RS19300	3878665..3879030	-	366	WP_197061372.1	STAS domain-containing protein	-
HM131_RS19305	3879027..3879896	-	870	WP_085031293.1	RsbT co-antagonist protein RsbRA	-
HM131_RS19310	3880131..3880481	-	351	WP_085032136.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HM131_RS19315	3880487..3880768	-	282	WP_035549809.1	hypothetical protein	Antitoxin
HM131_RS19320	3880927..3882081	-	1155	WP_085031294.1	alanine racemase	-
HM131_RS19325	3882423..3883442	-	1020	WP_085031295.1	outer membrane lipoprotein carrier protein LolA	-
HM131_RS19330	3883488..3885014	-	1527	WP_085031296.1	NAD(P)H-hydrate dehydratase	-
HM131_RS19335	3885073..3885426	-	354	WP_085031297.1	holo-ACP synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12947.05 Da
• Isoelectric Point: 7.0117

>T75411 WP_085032136.1 NZ_CP020772:c3880481-3880131 [Halobacillus mangrovi]
MIVKRGDVYFADLSPVVGSEQGGVRPVLILQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEINAEKYGFERNSVILLEQI
RTIDKQRLTDKITQLDDPMMQQVNKALQISLGLIDF

Nucleotide

• Length: 351 bp

>T75411 NZ_CP020772:c3880481-3880131 [Halobacillus mangrovi]
TTGATAGTCAAACGAGGAGACGTTTATTTCGCTGATTTGTCCCCGGTAGTGGGTTCAGAGCAAGGCGGGGTTCGTCCTGT
ACTCATTCTTCAAAATGATATCGGTAATCGTTTTAGCCCAACAGTAATCGTTGCAGCGATTACAGCGCAAATACAAAAAG
CCAAACTTCCAACTCACGTAGAAATTAACGCAGAAAAATACGGGTTTGAACGAAACTCTGTAATCCTATTGGAACAAATC
AGAACGATTGATAAACAGCGTTTGACCGACAAAATCACTCAACTTGATGATCCGATGATGCAACAAGTGAATAAAGCCTT
GCAGATCAGTCTCGGATTGATTGATTTTTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10798.30 Da
• Isoelectric Point: 4.4400

>AT75411 WP_035549809.1 NZ_CP020772:c3880768-3880487 [Halobacillus mangrovi]
MVLSDSMQEIVIRIPDNLLNEVDGFIQLENSDRSDFMCQATKMYLREKKQRHIRESMRRGYMEMAKINLNIASEAFQAEE
EAENTLEQLVGGV

Nucleotide

• Length: 282 bp

>AT75411 NZ_CP020772:c3880768-3880487 [Halobacillus mangrovi]
ATGGTTTTGTCCGACAGCATGCAGGAAATTGTCATCCGGATTCCGGATAACCTACTCAATGAAGTGGATGGCTTCATTCA
ACTCGAAAATAGTGATCGGAGCGATTTCATGTGTCAAGCAACTAAAATGTATTTGCGTGAAAAGAAACAACGCCACATTA
GAGAATCCATGCGTAGAGGCTATATGGAGATGGCGAAAATTAATTTAAACATCGCTTCAGAAGCGTTTCAAGCTGAAGAG
GAGGCAGAAAACACCCTGGAGCAATTAGTGGGCGGGGTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0B0D213