Detailed information of TA system TA075409

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/DUF433(antitoxin)
• Location: 4727845..4728404
• Replicon: chromosome
• Accession: NZ_CP020771
• Organism: Microcystis aeruginosa PCC 7806SL

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: BH695_RS23490
• Protein ID: WP_036399356.1
• Coordinates: 4727845..4728183 (-)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A1X9LGK3
• Locus tag: BH695_RS23495
• Protein ID: WP_002740734.1
• Coordinates: 4728180..4728404 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BH695_RS23465	4724507..4724971	+	465	WP_002738176.1	DNA phosphorothioation-associated protein 4	-
BH695_RS23470	4724980..4725618	-	639	WP_002740727.1	DUF1361 domain-containing protein	-
BH695_RS23475	4725622..4726038	-	417	WP_002740730.1	acyl-CoA thioesterase	-
BH695_RS23480	4726046..4726564	-	519	WP_002734244.1	cofactor assembly of complex C subunit B	-
BH695_RS23485	4726700..4727605	+	906	WP_002740732.1	metal ABC transporter permease	-
BH695_RS23490	4727845..4728183	-	339	WP_036399356.1	DUF5615 family PIN-like protein	Toxin
BH695_RS23495	4728180..4728404	-	225	WP_002740734.1	DUF433 domain-containing protein	Antitoxin
BH695_RS23500	4728892..4729116	+	225	WP_002740738.1	hypothetical protein	-
BH695_RS23505	4729605..4730048	-	444	WP_002740761.1	IS200/IS605 family transposase	-
BH695_RS23510	4730322..4730900	-	579	WP_002773172.1	hypothetical protein	-
BH695_RS23515	4731045..4731650	-	606	WP_036402960.1	DedA family protein	-
BH695_RS23520	4731748..4733025	-	1278	WP_002732258.1	adenosylhomocysteinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4729605..4730048	443

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Domain of unknown function DUF5615 (1-78)

Antitoxin

• Protein of unknown function DUF433 (8-63)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12331.14 Da
• Isoelectric Point: 4.1314

>T75409 WP_036399356.1 NZ_CP020771:c4728183-4727845 [Microcystis aeruginosa PCC 7806SL]
MTIWVDAHLSPAIATWISTTLEIEVVALRDLGLRDAEDTEIFQVAKAQRAILMTKDSDFVDLVERLGSPPQIIWLTCGNT
SNARLREILSETLPRALELLAAGETLVEISGD

Nucleotide

• Length: 339 bp

>T75409 NZ_CP020771:c4728183-4727845 [Microcystis aeruginosa PCC 7806SL]
ATGACAATTTGGGTTGACGCACATTTGTCTCCTGCAATTGCAACCTGGATTAGCACTACACTTGAGATTGAAGTTGTGGC
TTTGCGTGATCTTGGACTGAGAGATGCTGAAGATACTGAAATCTTTCAGGTTGCAAAAGCTCAAAGAGCTATCCTAATGA
CCAAAGACAGCGATTTTGTAGATTTAGTTGAGCGACTTGGTTCGCCCCCACAAATTATTTGGCTGACTTGTGGCAATACC
TCTAATGCCCGGTTGCGTGAAATCTTGAGTGAGACTTTACCCAGGGCTTTAGAACTTTTAGCTGCTGGTGAGACATTGGT
AGAAATTAGCGGAGACTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8134.55 Da
• Isoelectric Point: 6.9476

>AT75409 WP_002740734.1 NZ_CP020771:c4728404-4728180 [Microcystis aeruginosa PCC 7806SL]
MAELLSRITVNPRQCGGRACIRGMRIRVSDVLDLFAAGLSAREILDEMPDLEADDLKAALLYASRKLNHPVLLA

Nucleotide

• Length: 225 bp

>AT75409 NZ_CP020771:c4728404-4728180 [Microcystis aeruginosa PCC 7806SL]
ATGGCGGAATTGCTTTCGAGAATTACAGTTAATCCTCGGCAGTGCGGTGGTCGTGCCTGTATTCGAGGAATGAGAATCCG
GGTATCAGATGTGTTAGATCTATTCGCGGCAGGTTTAAGCGCGAGAGAAATTCTAGATGAAATGCCTGATCTTGAGGCAG
ACGATTTGAAAGCGGCGCTTTTATATGCCTCGCGGAAGCTTAATCACCCGGTTTTACTGGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A1X9LGK3