Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3553302..3553933 Replicon chromosome
Accession NZ_CP020753
Organism Shigella flexneri 1c strain Y394

Toxin (Protein)


Gene name hicA Uniprot ID A0A822PMR8
Locus tag B7485_RS18465 Protein ID WP_001259384.1
Coordinates 3553658..3553933 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag B7485_RS18460 Protein ID WP_000593555.1
Coordinates 3553302..3553661 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B7485_RS18435 (3549434) 3549434..3550378 + 945 WP_000947076.1 nickel ABC transporter permease subunit NikB -
B7485_RS18440 (3550375) 3550375..3551208 + 834 WP_001008974.1 nickel ABC transporter permease subunit NikC -
B7485_RS18445 (3551208) 3551208..3551972 + 765 WP_001136248.1 nickel import ATP-binding protein NikD -
B7485_RS18450 (3551969) 3551969..3552775 + 807 WP_000173660.1 nickel import ATP-binding protein NikE -
B7485_RS18455 (3552781) 3552781..3553182 + 402 WP_001190069.1 nickel-responsive transcriptional regulator NikR -
B7485_RS18460 (3553302) 3553302..3553661 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
B7485_RS18465 (3553658) 3553658..3553933 - 276 WP_001259384.1 type II toxin-antitoxin system HicA family toxin Toxin
B7485_RS18470 (3553992) 3553992..3555116 - 1125 WP_001216257.1 ABC transporter permease -
B7485_RS18475 (3555116) 3555116..3557851 - 2736 WP_000149173.1 ribosome-associated ATPase/putative transporter RbbA -
B7485_RS18480 (3557848) 3557848..3558915 - 1068 WP_000361470.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-81)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10146.89 Da        Isoelectric Point: 10.3748

>T75402 WP_001259384.1 NZ_CP020753:c3553933-3553658 [Shigella flexneri 1c]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
CDWLLSIGVKP

Download         Length: 276 bp

>T75402 NZ_CP020753:c3553933-3553658 [Shigella flexneri 1c]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
TGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT75402 WP_000593555.1 NZ_CP020753:c3553661-3553302 [Shigella flexneri 1c]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT75402 NZ_CP020753:c3553661-3553302 [Shigella flexneri 1c]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCAGCTGTTATTACCTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGTATTCAGGGGCTGCAAAAAGAAGGAG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A822PMR8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References