Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2995527..2996181 Replicon chromosome
Accession NZ_CP020753
Organism Shigella flexneri 1c strain Y394

Toxin (Protein)


Gene name cptA Uniprot ID Q0T101
Locus tag B7485_RS15600 Protein ID WP_000244767.1
Coordinates 2995527..2995934 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID Q0T100
Locus tag B7485_RS15605 Protein ID WP_000354044.1
Coordinates 2995915..2996181 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B7485_RS15580 (2991484) 2991484..2993217 - 1734 WP_000813187.1 single-stranded-DNA-specific exonuclease RecJ -
B7485_RS15585 (2993223) 2993223..2993933 - 711 WP_000715213.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
B7485_RS15590 (2993958) 2993958..2994854 - 897 WP_000806987.1 site-specific tyrosine recombinase XerD -
B7485_RS15595 (2994966) 2994966..2995487 + 522 WP_001055867.1 flavodoxin FldB -
B7485_RS15600 (2995527) 2995527..2995934 - 408 WP_000244767.1 protein YgfX Toxin
B7485_RS15605 (2995915) 2995915..2996181 - 267 WP_000354044.1 FAD assembly factor SdhE Antitoxin
B7485_RS15610 (2996424) 2996424..2997404 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
B7485_RS15615 (2997600) 2997600..2998259 - 660 WP_000250274.1 hemolysin III family protein -
B7485_RS15620 (2998423) 2998423..2998733 - 311 Protein_2993 N(4)-acetylcytidine aminohydrolase -
B7485_RS15625 (2998778) 2998778..3000211 + 1434 WP_141095330.1 6-phospho-beta-glucosidase BglA -
B7485_RS15630 (3000268) 3000268..3001011 - 744 WP_000951957.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15978.91 Da        Isoelectric Point: 10.9373

>T75400 WP_000244767.1 NZ_CP020753:c2995934-2995527 [Shigella flexneri 1c]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T75400 NZ_CP020753:c2995934-2995527 [Shigella flexneri 1c]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTATTTGATTGCG
TTCGCAGCCAGCGGTGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10529.09 Da        Isoelectric Point: 5.1599

>AT75400 WP_000354044.1 NZ_CP020753:c2996181-2995915 [Shigella flexneri 1c]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT75400 NZ_CP020753:c2996181-2995915 [Shigella flexneri 1c]
ATGGACATTAACAACAAAGCCCGAATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATAATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TIU0

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TIU2

References