Detailed information of TA system TA075375

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2527547..2528525
• Replicon: chromosome
• Accession: NZ_CP020743
• Organism: Bacillus mycoides strain Gnyt1

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: B7492_RS13315
• Protein ID: WP_085311113.1
• Coordinates: 2527547..2528287 (+)
• Length: 247 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: R8HWP4
• Locus tag: B7492_RS13320
• Protein ID: WP_000588712.1
• Coordinates: 2528400..2528525 (+)
• Length: 42 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7492_RS13290	2524114..2524824	+	711	WP_085311106.1	class I SAM-dependent methyltransferase	-
B7492_RS13295	2524943..2525356	+	414	WP_085311108.1	VOC family protein	-
B7492_RS13300	2525845..2526321	+	477	WP_085311110.1	MarR family winged helix-turn-helix transcriptional regulator	-
B7492_RS13305	2526475..2527212	+	738	WP_085311111.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
B7492_RS13315	2527547..2528287	+	741	WP_085311113.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
B7492_RS13320	2528400..2528525	+	126	WP_000588712.1	hypothetical protein	Antitoxin
B7492_RS35075	2528601..2528777	+	177	WP_170972692.1	hypothetical protein	-
B7492_RS13325	2528797..2529186	-	390	WP_085312972.1	YxeA family protein	-
B7492_RS13330	2529439..2530908	+	1470	WP_016102002.1	aminoacyl-histidine dipeptidase	-
B7492_RS35080	2531268..2531417	+	150	WP_002069316.1	hypothetical protein	-
B7492_RS13335	2531742..2533172	-	1431	WP_085311115.1	IS4 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2531742..2533172	1430

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-244)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 247 a.a.
• Molecular weight: 28471.19 Da
• Isoelectric Point: 8.2847

>T75375 WP_085311113.1 NZ_CP020743:2527547-2528287 [Bacillus mycoides]
MTISNIRIGLFILVIAFVVLVFFYWRNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIA
FILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPTAFGTMEWQTEEEYTKSLNAFLDSYGEKIGAK
IVVFEAVKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIH
HSYFSK

Nucleotide

• Length: 741 bp

>T75375 NZ_CP020743:2527547-2528287 [Bacillus mycoides]
TTGACGATCTCTAACATTCGAATTGGTTTATTTATTCTAGTAATCGCCTTTGTAGTTCTTGTTTTCTTTTATTGGAGAAA
TGAAGAGTTGTACGAGGAGAAGAAACAACGAATTCGAAAAACTTGGTATGGTTTGTTTATAATATCAGTCACAGTTTATT
TCATGATAAAAGGAATAGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCGATGGTAATTTTCGTTGATATTGCA
TTCATTTTGACACCAAATATTTCAGAAATATGGGGCGCAAAATTCAGCGATATTGGAAAGACGGTCCAATCAATTAAAAG
GTCATTAATTGCTTCAAAAGCAAGAGGAGAAATATATACGACAATTATTCAAAATGTAAATCCAACAGCCTTTGGGACAA
TGGAATGGCAAACGGAAGAGGAATATACAAAAAGCTTAAACGCATTTTTAGATTCATATGGGGAAAAGATTGGTGCGAAA
ATTGTTGTATTTGAAGCGGTGAAGGAATTAAATACAAACTTTCGTGGTATCCGCTCTCAATTTAGTATTATTGTTCCATT
AGAACACATCGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGAATTATACCAGCAAAAATAGTTAGTG
ATGTTTTCATTGTTATTGATGGGAAGAAAAATAACCTTCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATTCAT
CATAGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 42 a.a.
• Molecular weight: 4938.34 Da
• Isoelectric Point: 3.9150

>AT75375 WP_000588712.1 NZ_CP020743:2528400-2528525 [Bacillus mycoides]
VIKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRK

Nucleotide

• Length: 126 bp

>AT75375 NZ_CP020743:2528400-2528525 [Bacillus mycoides]
GTGATAAAAGATTATGACAATGATTTTTTTAAAGAAGATAATGAAGATACAACAGATTTCTCTTTAATAAAAGGAGATAC
TTTGCAGCAAGTAGAAGAGTTAGAAGAAGAATTAAAAAGAAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A366GQT1